CST, 13571S, Phospho-WIPI2 (Ser413) Antibody

Polyclonal Antibody for studying WIPI2 (Ser413) phosphate. Validated for Western Blotting,Immunoprecipitation. Highly specific and rigorously validated in-house, Phospho-WIPI2 (Ser413) Antibody (CST #13571) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:100 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity Phospho-WIPI2 (Ser413) Antibody recognizes endogenous levels of WIPI2 protein only when phosphorylated at Ser413. Species Reactivity: Human, Mouse, Rat Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser413 of human WIPI2 protein. Antibodies are purified by protein A and peptide affinity chromatography. Background Autophagy is a catabolic process for the autophagosomic-lysosomal degradation of bulk cytoplasmic contents (1,2). It is generally activated by conditions of nutrient deprivation but is also associated with a number of physiological processes, including development, differentiation, neurodegeneration, infection, and cancer (3). The molecular machinery of autophagy was largely discovered in yeast and is directed by a number of autophagy-related (Atg) genes. Vacuolar trafficking and autophagy are controlled by the class III type phosphoinositide 3-kinase (PI3K) Vps34, which generates phosphoinositide-3-phosphate (PtdIns3P) (4,5). Atg18 and Atg21 are two related WD-repeat proteins that bind PtdIns3P via a conserved Phe-Arg-Arg-Gly motif (6,7). It has been shown that Atg18 binds to Atg2 and that this complex is directed to vacuolar membranes by its interaction with PtdIns3P (8). Human orthologs of Atg18 and Atg21 were identified as members of the WD-repeat protein interacting with phosphoinositides (WIPI) family (9-11). WIPI1 (also called WIPI49) and WIPI2 have been shown to translocate from several vacuolar compartments to LC3-positive autophagosomes during autophagy; this translocation may be used as an autophagy marker (10). Phospho-WIPI2 (Ser413) Antibody is directed at a site that was identified at Cell Signaling Technology (CST) using PhosphoScan, CST's LC-MS/MS platform for modification site discovery. Phosphorylation at Ser413 was discovered using Phospho-MAPK/CDK Substrate motif antibody. Please visit PhosphoSitePlus, CST's modification site knowledgebase, at www.phosphosite.org for more information. Alternate Names ATG18B; Atg21; CGI-50; DKFZp434J154; DKFZp686P02188; FLJ12979; FLJ14217; FLJ42984; IDDSSA; WD repeat domain phosphoinositide-interacting protein 2; WD repeat domain, phosphoinositide interacting 2; WD40 repeat protein interacting with phosphoinositides 2; WIPI-2; WIPI2; WIPI49-like protein 2 Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 49 SOURCE: Rabbit