CST, 13589S, Phospho-Talin (Ser425) (D2P2M) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying Talin1 (Ser425) phosphate. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, Phospho-Talin (Ser425) (D2P2M) Rabbit Monoclonal Antibody (CST #13589) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity Phospho-Talin (Ser425) (D2P2M) Rabbit Monoclonal Antibody recognizes endogenous levels of talin protein only when phosphorylated at Ser425. Based on sequence homology, this antibody is expected to recognize both talin-1 and talin-2 proteins phosphorylated at Ser425. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser425 of human talin protein. Background Focal adhesions connect the cytoskeleton with the extracellular matrix (ECM), a complex structure of secreted macromolecules that surrounds mammalian organs and tissues. Integrins clustered on the extracellular side of focal adhesions signal from the ECM to intracellular protein complexes, which in turn signal to the actin cytoskeleton to regulate the tension needed for cell motility. Internal signals also converge on focal adhesions to regulate integrin affinity and avidity. Signaling through focal adhesions regulates cell adhesion, migration, proliferation, apoptosis, and gene expression, and impacts cellular processes such as development, wound healing, immune response, invasion, metastasis, and angiogenesis (reviewed in 1-3). Talin is a large, multidomain focal adhesion protein that interacts with the intracellular domains of integrins and other focal adhesion proteins. Talin is involved in the formation of focal adhesions and in linking focal adhesions to the actin cytoskeleton (4). The interaction between talin and integrins increases the affinity between integrin and both insoluble and soluble ECM proteins (5,6). Alternate Names ILWEQ; KIAA1027; talin 1; Talin-1; talin1; TLN; TLN1 Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 270 Source/Isotype: Rabbit IgG