CST, 13592T, Myosin VI (D5Y5A) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying MYO6. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, Myosin VI (D5Y5A) Rabbit Monoclonal Antibody (CST #13592) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity Myosin VI (D5Y5A) Rabbit Monoclonal Antibody recognizes endogenous levels of total myosin VI protein. Species Reactivity: Human, Dog Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Arg1131 of human myosin VI protein. Background The myosin family of motor proteins drive ATP-dependent actin-based motility in eukaryotic cells and contain a conserved amino-terminal motor domain (reviewed in 1,2). Myosin VI is an unconventional minus-end-directed myosin involved in the transport of vesicles and organelles within the cell, endocytosis, and organelle biogenesis (3-6). The movement of myosin VI and its cargo along actin filaments is unique among myosin family members in its mechanism; its tail domain structure allows it to take larger than predicted steps along the actin filament (reviewed in 1,7). Myosin VI has been shown to regulate the polarized delivery of proteins to specialized subcellular locations, including the delivery of EGFR to the leading edge of migrating cells (8), as well as the delivery of specialized axonal proteins in neurons (9). Myosin VI has also been shown to mediate activity of the tumor suppressor p53 during DNA damage (10,11). Alternate Names DFNA22; DFNB37; KIAA0389; MYO6; myosin VI; Myosin-VI; Unconventional myosin-6; Unconventional myosin-VI Specification REACTIVITY: H Dg SENSITIVITY: Endogenous MW (kDa): 150 Source/Isotype: Rabbit IgG