CST, 13609T, DNA Polymerase gamma (D1Y6R) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying POLG. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, DNA Polymerase gamma (D1Y6R) Rabbit Monoclonal Antibody (CST #13609) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity DNA Polymerase gamma (D1Y6R) Rabbit Monoclonal Antibody recognizes endogenous levels of total DNA polymerase γ protein. Species Reactivity: Human, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro560 of human DNA polymerase γ protein. Background DNA polymerase γ (POLG, pol gamma) is a nuclear encoded protein that is responsible for mitochondrial genome replication in eukaryotic cells. The 140 kDa polymerase γ catalytic subunit forms a holoenzyme complex with a 55 kDa accessory protein (POLG2, pol γB) dimer, which confers processivity (1). In addition to polymerase activity, polymerase γ contains 3'-5' exonuclease activity for proofreading and 5′-deoxyribonucleic phosphate lyase activity that functions in DNA base excision repair (BER). The rate at which the catalytic subunit recognizes damaged DNA during DNA repair is enhanced by the pol γB accessory subunit (2). Mutations in the corresponding gene are associated with several inherited neuropathies including progressive external ophthalmoplegia, myocerebrohepatopathy spectrum disorders and Alpers-Huttenlocher syndrome (3,4). Research studies indicate that mutations in the corresponding gene may promote breast tumorigenesis (5). Alternate Names DNA polymerase gamma, catalytic subunit; DNA polymerase subunit gamma-1; DNA-directed DNA polymerase gamma; DPOG1; FLJ27114; MDP1; MIRAS; Mitochondrial DNA polymerase catalytic subunit; mitochondrial polymerase gamma catalytic subunit; MTDPS4A; MTDPS4B; PEO; POLG; PolG-alpha; PolG, catalytic subunit; POLG1; POLGA; polymerase (DNA directed), gamma; polymerase (DNA) gamma, catalytic subunit; SANDO; SCAE; truncated mitochondrial DNA polymerase gamma catalytic subunit; truncated mitochondrial polymerase gamma catalytic subunit Specification REACTIVITY: H Mk SENSITIVITY: Endogenous MW (kDa): 140 Source/Isotype: Rabbit IgG