CST, 13613T, NUP88 (D7U4O) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying NUP88. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, NUP88 (D7U4O) Rabbit Monoclonal Antibody (CST #13613) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity NUP88 (D7U4O) Rabbit Monoclonal Antibody recognizes endogenous levels of total NUP88 protein. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly676 of human NUP88 protein. Background The nuclear pore complex (NPC) is a multi-subunit protein channel that spans the nuclear envelope and is responsible for the nucleocytoplasmic trafficking of RNA, proteins, and ribonucleoproteins (1). The 88 kDa nucleoporin protein (NUP88) is located on the cytoplasmic side of the NPC and found to be a part of a subcomplex with the NUP214 NPC subunit (1-3). This NUP88/NUP214 subcomplex interacts with CRM-1/exportin-1 to play an important role in the export of proteins, including the 60S ribosomal particle from the nucleus (4,5). Research studies demonstrate elevated expression and cytoplasmic accumulation of NUP88, visible as granular dots, in ovarian, prostate, and breast cancers (6-9). Increased NUP88 levels correlate with higher tumor grade, suggesting that NUP88 may be a putative tumor marker (1,7). Alternate Names 88 kDa nucleoporin; FADS4; karyoporin; MGC8530; nuclear pore complex protein 88; Nuclear pore complex protein Nup88; nucleoporin 88; nucleoporin 88kDa; Nucleoporin Nup88; NUP88 Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 88 Source/Isotype: Rabbit IgG