{"product_id":"cst-13621s","title":"CST,  13621S, SB216763","description":"Chemical Modulators for studying in the research area.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nSB216763 is supplied as a lyophilized powder. For a 25 mM stock, reconstitute the 5 mg in 538.8 µl DMSO. Working concentrations and length of treatment can vary depending on the desired effect, but it is typically used at 5-25 µM for 3-24 hr.\n\u003cb\u003eStorage\u003c\/b\u003e\nStore lyophilized or in solution at -20ºC, desiccated. In lyophilized form, the chemical is stable for 24 months. Once in solution, use within 3 months to prevent loss of potency. Aliquot to avoid multiple freeze\/thaw cycles.\n\u003cb\u003eBackground\u003c\/b\u003e\nThe maleimide derivative SB216763 is a potent and selective cell permeable inhibitor of glycogen synthase kinase 3 (GSK-3). Research studies using peptide-based protein kinase assays show that SB216763 inhibits GSK-3α in an ATP competitive manner with an IC of 34 nM, and is an equally effective GSK3-β inhibitor. Similar assays demonstrate that SB216763 (at concentrations up to 10 µM) does not inhibit as many as 24 other serine\/threonine and tyrosine protein kinases (1). As a consequence of inhibiting GSK-3, SB216763 stimulates glycogen synthesis in human liver cells (EC 3.6 µM) via glycogen synthase activation and induces expression of a β-catenin regulated reporter gene in HEK293 cells (1). Furthermore, SB216763 induces accumulation of β-catenin, a key downstream effector in the Wnt signaling pathway, in many cell types (2-5). Additional research indicates that SB216763 can prevent neuronal cell death induced by PI3 kinase pathway inhibition (2). Glycogen synthase kinase 3 inhibitors such as SB216763 can be important research tools in studying the functional role of GSK-3 in cell signaling pathways.\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nakt; beta; catenin; glycogen synthase; GSK; inhibitor; insulin; PI3","brand":"CST","offers":[{"title":"Default Title","offer_id":46800692183209,"sku":"13621S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-13621s","provider":"Iright","version":"1.0","type":"link"}