CST, 13662S, VCAM-1 (E1E8X) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying VCAM1. Validated for Western Blotting,Immunoprecipitation,Flow Cytometry (Live). Available in 2 sizes. Highly specific and rigorously validated in-house, VCAM-1 (E1E8X) Rabbit Monoclonal Antibody (CST #13662) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:100 Flow Cytometry (Live): 1:50 - 1:200 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation, Flow Cytometry (Live) Specificity / Sensitivity VCAM-1 (E1E8X) Rabbit Monoclonal Antibody recognizes endogenous levels of total VCAM-1 protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro354 of human VCAM-1 protein. Background VCAM-1 (vascular cell adhesion molecule-1) is a transmembrane glycoprotein containing multiple amino-terminal extracellular Ig-like domains, a transmembrane domain, and a short carboxy-terminal cytoplasmic domain (1). Alternative splicing generates two isoforms of VCAM-1 (2). The role of VCAM-1 during infection and inflammatory diseases is well characterized. Expression of VCAM-1 is induced in endothelial cells by inflammatory cytokines including TNF-α and IL-1β (1). VCAM-1 on endothelial cells interacts with the integrin VLA-4 (α4β1) on leukocytes to mediate migration of circulating leukocytes from the blood across the endothelium and into tissues (3). Alternate Names CD106; CD106 antigen; DKFZp779G2333; INCAM-100; L1CAM; MGC99561; V-CAM 1; vascular cell adhesion molecule 1; Vascular cell adhesion protein 1; VCAM-1; VCAM1 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 95-120 Source/Isotype: Rabbit IgG