CST, 13689S, TRIAD1 Antibody

Polyclonal Antibody for studying ARIH2. Validated for Western Blotting. Highly specific and rigorously validated in-house, TRIAD1 Antibody (CST #13689) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity TRIAD1 Antibody recognizes endogenous levels of total TRIAD1 protein. Based upon sequence alignment, this antibody is not predicted to cross-react with HHARI/ARIH1. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human TRIAD1 protein. Antibodies are purified by protein A and peptide affinity chromatography. Background The E3 ubiquitin-protein ligase ARIH2 (TRIAD1) is an Ariadne subfamily ligase involved in the polyubiquitination of proteins designated for proteasomal degradation. The TRIAD1 nuclear protein contains an amino-terminal acidic region, a pair of RING fingers, two carboxyl-terminal coiled coil domains and a novel C6HC DRIL/IBR domain located between the RING fingers. Together, the paired RING fingers and DRIL/IBR domain form a highly conserved TRIAD (two RING fingers and DRIL) domain (1). Research studies suggest that TRIAD1 mediates both Lys48 and Lys63 protein polyubiquitination and acts as a negative regulator of myelopoiesis. TRIAD1 ubiquitin ligase inhibits myeloid cell proliferation by mediating protein ubiquitination through the ubiquitin-conjugating enzymes UbcH7 and UbcH13 (2,3). Experimental deletion of TRIAD1 in mice has a lethal effect, leading to death at the embryonic stage or later due to a severe, multi-organ inflammatory response. Results indicate that TRIAD1 binds IκBβ in dendritic cells and promotes the degradation of the NF-κB inhibitor (4). Alternate Names all-trans retinoic acid inducible RING finger; ARI-2; ARI2; ariadne homolog 2; ariadne homolog 2 (Drosophila); ariadne RBR E3 ubiquitin protein ligase 2; ARIH2; E3 ubiquitin-protein ligase ARIH2; FLJ10938; FLJ33921; Protein ariadne-2 homolog; RING-type E3 ubiquitin transferase ARIH2; TRIAD1; Triad1 protein Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 58 SOURCE: Rabbit