{"product_id":"cst-13703s","title":"CST,  13703S, Phospho-DAPP1\/BAM32 (Tyr139) (D7G4G) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying DAPP1\/BAM32 (Tyr139) phosphate. Validated for Western Blotting,Simple Western™,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, Phospho-DAPP1\/BAM32 (Tyr139) (D7G4G) Rabbit Monoclonal Antibody (CST #13703) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\nSimple Western™: 1:50 - 1:250\nImmunoprecipitation: 1:200\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Immunoprecipitation\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nPhospho-DAPP1\/BAM32 (Tyr139) (D7G4G) Rabbit Monoclonal Antibody recognizes endogenous levels of DAPP1\/BAM32 protein only when phosphorylated at Tyr139.\nSpecies Reactivity: Human\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr139 of human DAPP1\/BAM32 protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nThe dual adaptor of phosphotyrosine and 3-phosphoinositides (DAPP1\/BAM32) is a cytoplasmic adaptor protein that mediates the recruitment and interaction of molecules required for signal transduction downstream of the B cell receptor (BCR) (1). The DAPP1\/BAM32 protein contains an amino-terminal SH2 domain and a carboxy-terminal pleckstrin homology (PH) domain that binds to PI3K-derived phosphoinositides (i.e., PIP ). Upon BCR activation, DAPP1\/BAM32 is phosphorylated at specific tyrosine residues and translocated from the cytoplasm to the membrane. Research studies indicate that phosphorylation and translocation of DAPP1\/BAM32 is strongly dependent upon PI3K signaling (2,3). The amino-terminal SH2 domain binds to PLCγ2 and other tyrosine-phosphorylated targets. As a result of these interactions, DAPP1\/BAM32 can adjust the response to receptor activation by coordinating membrane-localized interactions among proteins of distinct signal transduction pathways (1,4). DAPP1\/BAM32 is expressed most abundantly in B lymphocytes; high expression during dendritic cell (DC) maturation and localization to contact sites between DC and allogenic T cells suggest that the DAPP1\/BAM32 adaptor may play a role in the activation of T cells through MHC class I-mediated signaling pathways (5). Research studies show that phosphorylation of DAPP1\/BAM32 at Tyr139 is PI3K-dependent, requires an intact PH domain in DAPP1\/BAM32, and is likely performed by Src-family kinases following membrane recruitment of DAPP1\/BAM32 by phosphoinositides (6). Blocking phosphorylation of DAPP1\/BAM32 at Tyr139 inhibits BCR internalization and reduces cellular F-actin levels, suggesting that phosphorylation of DAPP1\/BAM32 may play a role in regulating actin-dependent internalization of the activated BCR (7,8).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nB lymphocyte adapter protein Bam32; B-cell adapter molecule of 32 kDa; BAM32; DAPP1; DKFZp667E0716; Dual adapter for phosphotyrosine and 3-phosphotyrosine and 3-phosphoinositide; dual adaptor of phosphotyrosine and 3-phosphoinositides; dual adaptor of phosphotyrosine and 3-phosphoinositides 1; hDAPP1\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H\nSENSITIVITY: Endogenous\nMW (kDa): 29\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46797599408297,"sku":"13703S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-13703s","provider":"Iright","version":"1.0","type":"link"}