{"product_id":"cst-13726t","title":"CST,  13726T, PSMB8\/LMP7 (1A5) Mouse Monoclonal Antibody","description":"Monoclonal Antibody for studying PSMB8. Validated for Western Blotting,Immunofluorescence (Immunocytochemistry),Flow Cytometry (Fixed\/Permeabilized). Available in 2 sizes. Highly specific and rigorously validated in-house, PSMB8\/LMP7 (1A5) Mouse Monoclonal Antibody (CST #13726) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\nImmunofluorescence (Immunocytochemistry): 1:50\nFlow Cytometry (Fixed\/Permeabilized): 1:50\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 60347 .\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Immunofluorescence (Immunocytochemistry), Flow Cytometry (Fixed\/Permeabilized)\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nPSMB8\/LMP7 (1A5) Mouse Monoclonal Antibody recognizes endogenous levels of total PSMB8\/LMP7 protein. This antibody recognizes both 28 kDa precursor and 23 kDa mature forms of PSMB8\/LMP7 and does not cross-react with PSMB5 protein.\nSpecies Reactivity: Human, Rat\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with recombinant protein encompassing the full-length of human PSMB8\/LMP7 protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nThe 26S proteasome is a highly abundant proteolytic complex involved in the degradation of ubiquitinated substrate proteins. It consists largely of two sub-complexes, the 20S catalytic core particle (CP) and the 19S\/PA700 regulatory particle (RP) that can cap either end of the CP. The CP consists of two stacked heteroheptameric β-rings (β ) that contain three catalytic β-subunits and are flanked on either side by two heteroheptameric α-rings (α ). The RP includes a base and a lid, each having multiple subunits. The base, in part, is composed of a heterohexameric ring of ATPase subunits belonging to the AAA (ATPases Associated with diverse cellular Activities) family. The ATPase subunits function to unfold the substrate and open the gate formed by the α-subunits, thus exposing the unfolded substrate to the catalytic β-subunits. The lid consists of ubiquitin receptors and DUBs that function in recruitment of ubiquitinated substrates and modification of ubiquitin chain topology (1,2). Other modulators of proteasome activity, such as PA28\/11S REG, can also bind to the end of the 20S CP and activate it (1,2). Constitutively expressed core particle subunits PSMB5, PSMB7, and PSMB6 provide chymotrypsin-like, trypsin-like, and caspase-like activities, respectively (3). In immune cells involved in antigen presentation, these subunits are replaced by highly homologous, induced β-subunits to form the immunoproteasome (4,5). Proteasome subunit beta type-8 (PSMB8, LMP7) is expressed as a proenzyme that is cleaved to form the mature PSMB8 (LMP7) immunoproteasome core particle subunit (6). Interferon-γ induces expression of PSMB8, which functionally replaces the PSMB5 core particle subunit in immunoproteasome processing of MHC class I-restricted peptide antigens (7). Research studies suggest that reduced PSMB8 expression or expression of the non-functional LMP7-E1 isoform may impair immunoproteasome assembly, and that PSMB8 deficiency results in reduced MHC class I molecule expression (8-10). Inhibition of PSMB8 in murine rheumatoid arthritis models attenuates disease indicators, suggesting that PSMB8 is a potential therapeutic target in the treatment of some proinflammatory autoimmune diseases (11). Mutations in the corresponding PSMB8 gene can cause an autoinflammatory syndrome known as CANDLE Syndrome (12).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nALDD; D6S216; D6S216E; JMP; LMP7; Low molecular mass protein 7; low molecular weight protein 7; Macropain subunit C13; MGC1491; Multicatalytic endopeptidase complex subunit C13; NKJO; PRAAS1; protease component C13; proteasome (prosome, macropain) subunit, beta type, 8 (large multifunctional peptidase 7); proteasome 20S subunit beta 8; proteasome catalytic subunit 3i; Proteasome component C13; proteasome subunit beta 5i; proteasome subunit beta 8; Proteasome subunit beta type-8; Proteasome subunit beta-5i; proteasome subunit Y2; proteasome-related gene 7; PSB8; PSMB5i; PSMB8; Really interesting new gene 10 protein; RING10; Y2\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H R\nSENSITIVITY: Endogenous\nMW (kDa): 23, 28\nSource\/Isotype: Mouse IgG1","brand":"CST","offers":[{"title":"Default Title","offer_id":46797599735977,"sku":"13726T","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-13726t","provider":"Iright","version":"1.0","type":"link"}