CST, 13812T, MyoD1 (D8G3) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying MyoD. Validated for Western Blotting,Immunofluorescence (Immunocytochemistry). Available in 2 sizes. Highly specific and rigorously validated in-house, MyoD1 (D8G3) Rabbit Monoclonal Antibody (CST #13812) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunofluorescence (Immunocytochemistry): 1:400 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunofluorescence (Immunocytochemistry) Specificity / Sensitivity MyoD1 (D8G3) Rabbit Monoclonal Antibody recognizes endogenous levels of total MyoD1 protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly190 of human MyoD1 protein. Background Myoblast determination protein 1 (MyoD1), also called myogenic factor 3 (Myf3), is a member of the MyoD family of muscle specific bHLH transcription factors (1). This family is responsible for controlling Specification of the muscle cell lineage and members are expressed only in skeletal muscle and its precursors. MyoD1 is considered a master regulator of skeletal myogenesis as its expression can induce myogenic differentiation in myoblasts, fibroblasts, and a variety of other cell types (2,3). Through ChIP-sequencing experiments, researchers have discovered that MyoD is associated with the promoters of many genes in muscle cells, but it only regulates a subset of those genes. These research studies point to regulation of MyoD transcriptional activity via epigenetic mechanisms involving SWI/SNF complexes and Polycomb and Trithorax Group proteins (4-6). Additional influences on muscle development include signal transduction through MAPK, PI3K/Akt, myostatin, NF-κB, and mTOR signaling pathways (5-7). Alternate Names BHLHC1; Class C basic helix-loop-helix protein 1; Myf-3; MYF3; Myoblast determination protein 1; MYOD; MYOD1; MYODRIF; myogenic differentiation 1; Myogenic factor 3; PUM Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 45 Source/Isotype: Rabbit IgG