{"product_id":"cst-13812t","title":"CST,  13812T, MyoD1 (D8G3) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying MyoD. Validated for Western Blotting,Immunofluorescence (Immunocytochemistry). Available in 2 sizes. Highly specific and rigorously validated in-house, MyoD1 (D8G3) Rabbit Monoclonal Antibody (CST #13812) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\nImmunofluorescence (Immunocytochemistry): 1:400\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Immunofluorescence (Immunocytochemistry)\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nMyoD1 (D8G3) Rabbit Monoclonal Antibody recognizes endogenous levels of total MyoD1 protein.\nSpecies Reactivity: Human\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly190 of human MyoD1 protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nMyoblast determination protein 1 (MyoD1), also called myogenic factor 3 (Myf3), is a member of the MyoD family of muscle specific bHLH transcription factors (1). This family is responsible for controlling \u003cb\u003eSpecification\u003c\/b\u003e of the muscle cell lineage and members are expressed only in skeletal muscle and its precursors. MyoD1 is considered a master regulator of skeletal myogenesis as its expression can induce myogenic differentiation in myoblasts, fibroblasts, and a variety of other cell types (2,3). Through ChIP-sequencing experiments, researchers have discovered that MyoD is associated with the promoters of many genes in muscle cells, but it only regulates a subset of those genes. These research studies point to regulation of MyoD transcriptional activity via epigenetic mechanisms involving SWI\/SNF complexes and Polycomb and Trithorax Group proteins (4-6). Additional influences on muscle development include signal transduction through MAPK, PI3K\/Akt, myostatin, NF-κB, and mTOR signaling pathways (5-7).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nBHLHC1; Class C basic helix-loop-helix protein 1; Myf-3; MYF3; Myoblast determination protein 1; MYOD; MYOD1; MYODRIF; myogenic differentiation 1; Myogenic factor 3; PUM\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H\nSENSITIVITY: Endogenous\nMW (kDa): 45\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46797600751785,"sku":"13812T","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-13812t","provider":"Iright","version":"1.0","type":"link"}