CST, 13824S, CDIP Antibody

Polyclonal Antibody for studying CDIP. Validated for Western Blotting,Immunoprecipitation. Highly specific and rigorously validated in-house, CDIP Antibody (CST #13824) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity CDIP Antibody recognizes endogenous levels of total CDIP protein. This antibody may detect proteins of unknown origin at 35 and 70 kDa in some cell lines. Species Reactivity: Human Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human CDIP protein. Antibodies are purified by protein A and peptide affinity chromatography. Background Cell Death Involved p53-Target (CDIP) is a pro-apoptotic protein induced by genotoxic stress in a p53-dependent manner (1). Indeed, CDIP is a direct target of p53. Inhibition of CDIP impairs p53-mediated apoptosis. Induction of apoptosis by CDIP requires caspase-8 suggesting involvement in the extrinsic apoptotic pathway. CDIP also promotes increased expression of TNF-α capable of triggering apoptosis. Furthermore, expression of CDIP correlates with increased sensitivity of TNF-α-induced apoptosis (2). These studies suggest that CDIP may serve as a marker for apoptotic sensitivity. Alternate Names 5730403B10Rik; C16orf5; CDIP; CDIP1; cell death inducing p53 target 1; cell death inducing protein; Cell death involved p53-target; Cell death-inducing p53-target protein 1; Cell death-inducing protein; chromosome 16 open reading frame 5; I1; Lipopolysaccharide-induced tumor necrosis factor-alpha-like protein; LITAF-like protein; LITAFL; LITFL; Transmembrane protein I1 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 24,26 SOURCE: Rabbit