CST, 13879S, Rheb (E1G1R) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying Rheb. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, Rheb (E1G1R) Rabbit Monoclonal Antibody (CST #13879) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 53730 . Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity Rheb (E1G1R) Rabbit Monoclonal Antibody recognizes endogenous levels of total Rheb protein. This antibody does not cross-react with RhebL1 protein. Bands of unknown origin may be detected at 30 kDa and 50 kDa in some cell lines. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu165 of human Rheb protein. Background Ras Homolog Enriched in Brain (Rheb) is an evolutionarily conserved member of the Ras family of small GTP-binding proteins originally found to be rapidly induced by synaptic activity in the hippocampus following seizure (1). While it is expressed at relatively high levels in the brain, Rheb is widely expressed in other tissues and may be induced by growth factor stimulation. Like other Ras family members, Rheb triggers activation of the Raf-MEK-MAPK pathway (2). Biochemical and genetic studies demonstrate that Rheb has an important role in regulating the insulin/TOR signaling pathway (3-6). The mammalian target of rapamycin (mTOR) is a serine/threonine protein kinase that acts as a sensor for ATP and amino acids, balancing the availability of nutrients with translation and cell growth. The tuberin/hamartin (TSC2/TSC1) complex inhibits mTOR activity indirectly by inhibiting Rheb through the tuberin GAP activity (7). Alternate Names GTP-binding protein Rheb; MGC111559; Ras homolog enriched in brain; Ras homolog enriched in brain 2; Ras homolog, mTORC1 binding; RHEB; RHEB2 Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 16 Source/Isotype: Rabbit IgG