CST, 13885T, Plac8 (E1J2Z) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying Plac8. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, Plac8 (E1J2Z) Rabbit Monoclonal Antibody (CST #13885) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity Plac8 (E1J2Z) Rabbit Monoclonal Antibody recognizes endogenous levels of total Plac8 protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro19 of human Plac8 protein. Background The placenta-specific gene 8 ( ) encodes a chromatin-binding protein that was first identified from placenta and that is highly expressed in plasmacytoid dendritic cells (1,2). Research studies indicate that Plac8 plays a role in immune function and is essential in the differentiation of brown fat (3,4). During brown fat differentiation, Plac8 interacts with the transcription factor C/EBPβ at its promoter sequence to activate the transcription of C/EBPβ (4). Experimental deletion of Plac8 in mice leads to impaired innate immune function, abnormal brown fat function, cold intolerance, and obesity (3,4). Additional studies examining the spread of colorectal carcinoma suggest a possible role for Plac8 in cancer invasion, possibly through promoting the epithelial-to-mesenchymal transition seen during tumor progression (5). Alternate Names C15; DGIC; down-regulated in gastrointestinal cancer protein; onzin; PLAC8; placenta associated 8; placenta specific 8; placenta-specific 8; Placenta-specific gene 8 protein; PNAS-144; Protein C15 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 13 Source/Isotype: Rabbit IgG