{"product_id":"cst-13901l","title":"CST,  13901L, Vinculin (E1E9V) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying vinculin. Validated for WB,WB,IHC,IHC,F. Available in 3 sizes. Highly specific and rigorously validated in-house, Vinculin (E1E9V) Rabbit Monoclonal Antibody (CST #13901) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\nSimple Western™: 1:10 - 1:50\nIHC Leica Bond: 1:600 - 1:2400\nImmunohistochemistry (Paraffin): 1:150 - 1:600\nFlow Cytometry (Fixed\/Permeabilized): 1:400 - 1:1600\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody . For a carrier free (BSA and azide free) version of this product see product # 40211 .\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, IHC Leica Bond, Immunohistochemistry (Paraffin), Flow Cytometry (Fixed\/Permeabilized)\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nVinculin (E1E9V) Rabbit Monoclonal Antibody recognizes endogenous levels of total vinculin protein. This antibody also reacts with metavinculin, a 145 kDa splice variant of vinculin.\nSpecies Reactivity: Human, Mouse, Rat, Monkey\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with recombinant protein specific to the amino terminus of human vinculin protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nVinculin is a cytoskeletal protein that plays an important role in the regulation of focal adhesions and embryonic development (1-4). Three structural vinculin domains include an amino-terminal head, a short, flexible proline-rich region, and a carboxy-terminal tail (1). In the inactive state, the head and tail domains of vinculin interact to form a closed conformation. The open and active form of vinculin translocates to focal adhesions, where it is thought to be involved in anchoring F-actin to the membrane and regulation of cell migration (2). Phospholipid binding to the tail domain and subsequent phosphorylation of vinculin at Ser1033 and Ser1045 by PKC-α and Tyr100 and Tyr1065 by Src kinases weakens the head-tail interaction (5,6). This change in vinculin allows the binding of a number of other proteins, including talin, α-actinin, and paxillin, which disrupts the head-tail interaction and initiates the conformational change from the inactive to active state (2,4). Vinculin deficiencies are associated with a decrease in cell adhesion and an increase in cell motility, suggesting a possible role in metastatic growth (7,8). This is supported by a demonstrated relationship between decreased vinculin expression and increased carcinogenesis and metastasis in colorectal carcinoma (9).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nCMD1W; CMH15; epididymis luminal protein 114; epididymis secretory sperm binding protein; HEL114; meta-vinculin; Metavinculin; MV; MVCL; VCL; VINC; Vinculin; Vinculin iso2\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H M R Mk\nSENSITIVITY: Endogenous\nMW (kDa): 124\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46797601865897,"sku":"13901L","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-13901l","provider":"Iright","version":"1.0","type":"link"}