{"product_id":"cst-13916s","title":"CST,  13916S, eRF1 Antibody","description":"Polyclonal Antibody for studying ETF1. Validated for Western Blotting. Highly specific and rigorously validated in-house, eRF1 Antibody (CST #13916) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\neRF1 Antibody recognizes endogenous levels of total eRF1 protein.\nSpecies Reactivity: Human, Mouse, Rat, Monkey\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nPolyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Phe117 of human eRF1 protein. Antibodies are purified by protein A and peptide affinity chromatography.\n\u003cb\u003eBackground\u003c\/b\u003e\nEfficient termination of mRNA translation in eukaryotes is dependent upon a complex of two polypeptide release factors, eRF1 and eRF3 (1). The eukaryotic translation termination factor 1 (eRF1, ETF1) structurally resembles tRNA, which allows it to participate in stop codon recognition as well as hydrolysis of the peptidyl-tRNA conjugate (2,3). The eRF1 protein contains three functionally distinct domains, including an amino-terminal domain that harbors discrete motifs that participate in stop codon recognition (4,5). Lysine hydroxylation within the amino-terminal domain is required for efficient termination of mRNA translation (6). The central region of eRF1 harbors a GGQ motif that facilitates hydrolysis of peptidyl-tRNA conjugates (7), while its carboxy terminus participates in eRF3 binding (8).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nD5S1995; ERF; ERF1; ETF1; Eukaryotic peptide chain release factor subunit 1; Eukaryotic release factor 1; eukaryotic translation termination factor 1; MGC111066; polypeptide chain release factor 1; Protein Cl1; RF1; sup45 (yeast omnipotent suppressor 45) homolog-like 1; SUP45L1; TB3-1\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H M R Mk\nSENSITIVITY: Endogenous\nMW (kDa): 50\nSOURCE: Rabbit","brand":"CST","offers":[{"title":"Default Title","offer_id":46797602291881,"sku":"13916S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-13916s","provider":"Iright","version":"1.0","type":"link"}