CST, 13974T, LMAN1 (E2B6H) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying ERGIC53. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, LMAN1 (E2B6H) Rabbit Monoclonal Antibody (CST #13974) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity LMAN1 (E2B6H) Rabbit Monoclonal Antibody recognizes endogenous levels of total LMAN1 protein. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu366 of human LMAN1 protein. Background Mannose-binding lectin-1 (LMAN1, ERGIC-53) is a type I transmembrane lectin protein localized to the intermediate compartment between the endoplasmic reticulum and the Golgi body (ERGIC) (1). Interaction between the LMAN1 protein and MCFD2 forms an ERGIC cargo receptor that delivers proteins from the ER to the Golgi body (2,3). The LMAN1 protein contains an amino-terminal carbohydrate recognition domain (CRD) that binds target glycoproteins, a membrane proximal oligomerization domain required for cargo transport, a single transmembrane segment, and short cytoplasmic tail (3-5). LMAN1 functions as a cargo receptor responsible for transport of glycoproteins from the ER to ERGIC and Golgi body. Target proteins include coagulation factors V and VIII, cathepsin C, cathepsin Z, and α1-antitrypsin (6-8). Mutations in the corresponding gene can result in combined factors FV and FVIII deficiency, an autosomal recessive disorder characterized by spontaneous bleeding (9). Inactivating frameshift mutations in are found at high frequency in colorectal tumors with microsatellite instability and may contribute to tumorigenesis (10). Alternate Names endoplasmic reticulum-golgi intermediate compartment protein 53; ER-Golgi intermediate compartment 53 kDa protein; ERGIC-53; ERGIC53; F5F8D; FMFD1; Gp58; intracellular mannose specific lectin; Intracellular mannose-specific lectin MR60; Lectin mannose-binding 1; lectin, mannose binding 1; lectin, mannose-binding, 1; LMAN1; MCFD1; MR60; Protein ERGIC-53 Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 53 Source/Isotype: Rabbit IgG