CST, 13996S, Viperin (D5T2X) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying Viperin. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, Viperin (D5T2X) Rabbit Monoclonal Antibody (CST #13996) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:100 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity Viperin (D5T2X) Rabbit Monoclonal Antibody recognizes endogenous levels of total viperin protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val270 of human viperin protein. Background The antiviral protein viperin (RSAD2) is induced by viral infection, lipopolysaccharides (LPS), polyriboinosinic polyribocytidylic acid [poly(I:C)], and interferons (1,2). Viperin protein localizes to the ER, redistributing to the Golgi and then to lipid droplets following viral infection (1,3). Viruses are known to use lipid droplets for replication, and the localization of the antiviral viperin protein to these lipid droplets is likely part of a cellular mechanism to inhibit these pathogens (4). Research studies indicate that HIV induction of viperin in human macrophages inhibits virus production and that siRNA targeting viperin reduces inhibition of HIV replication observed in poly(I:C) treated astrocytes (5,6). Additional research suggests that human cytomegalovirus (HCMV) co-opts viperin protein function, resulting in an interaction between viperin and the viral protein vMIA. This association leads to viperin relocalization to mitochondria, disrupting ATP generation and the actin cytoskeleton and increasing viral infection (7). The viperin protein also contributes to innate immune signaling by recruiting IRAK1 and TRAF6 to lipid droplets, resulting in IRF7 activation and type I interferon induction (8). Alternate Names 2510004L01Rik; cig33; CIG5; Cytomegalovirus-induced gene 5 protein; radical S-adenosyl methionine domain containing 2; Radical S-adenosyl methionine domain-containing protein 2; RSAD2; S-adenosylmethionine-dependent nucleotide dehydratase RSAD2; vig1; Viperin; Virus inhibitory protein, endoplasmic reticulum-associated, interferon-inducible Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 42 Source/Isotype: Rabbit IgG