CST, 14025S, PRNP (D3Q5C) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying PRNP. Validated for Western Blotting,Immunoprecipitation,Immunofluorescence (Immunocytochemistry). Available in 2 sizes. Highly specific and rigorously validated in-house, PRNP (D3Q5C) Rabbit Monoclonal Antibody (CST #14025) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Immunofluorescence (Immunocytochemistry): 1:200 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunofluorescence (Immunocytochemistry) Specificity / Sensitivity PRNP (D3Q5C) Rabbit Monoclonal Antibody recognizes endogenous levels of total PRNP protein. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser222 of human PRNP protein. Background The gene encodes the major prion protein (PrP, CD230), a widely-expressed glycoprotein expressed at high levels in the central nervous system (1). While the typical cellular function of PrP is not well defined, it is a putative antioxidant and a metal-binding protein that may be involved in signal transduction (2). Prion proteins can adopt different conformations; the cellular PrPc prion protein may be converted following translation into the β-sheet-rich scrapie isoform (PrPsc) responsible for several prion diseases, including bovine spongiform encephalopathy and human Creutzfeldt-Jakob disease (3). Unlike most neurodegenerative diseases, prion diseases are infectious as prions are capable of propagating by conferring an abnormally folded state onto properly folded cellular proteins (3). In addition, the cellular PrPc has may be involved in β-amyloid peptide oligomerization and synaptic toxicity (4). Alternate Names alternative prion protein; ALTPRP; ASCR; CD230; CD230 antigen; CJD; GSS; KURU; Major prion protein; MGC26679; p27-30; PRIO; prion; prion protein; prion protein PrP; prion-related protein; PRIP; PRNP; PRP; PrP27-30; PrP33-35C; PrPc Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 20-40 Source/Isotype: Rabbit IgG