CST, 14032T, GPR50 (D1D6I) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying GPR50. Validated for Western Blotting,Immunoprecipitation,Immunofluorescence (Immunocytochemistry). Available in 2 sizes. Highly specific and rigorously validated in-house, GPR50 (D1D6I) Rabbit Monoclonal Antibody (CST #14032) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Immunofluorescence (Immunocytochemistry): 1:400 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunofluorescence (Immunocytochemistry) Specificity / Sensitivity GPR50 (D1D6I) Rabbit Monoclonal Antibody recognizes endogenous levels of total GPR50 protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro420 of human GPR50 protein. Background G-protein coupled receptor 50 (GPR50) is an orphan G-protein coupled receptor with high sequence homology to the melatonin receptors MT1 and MT2. While classified as a member of the melatonin receptor family and also known as melatonin-related receptor, GPR50 does not bind melatonin (1). GPR50 forms heterodimers with MT1 and MT2 and acts as a negative regulator of MT1 agonist binding and G protein coupling; inhibition of melatonin receptor activity is dependent on the long, proline-rich carboxy terminal tail of GPR50 (2). On a physiological level, GPR50 is involved in the regulation of adaptive thermoregulation in mammals and deletion of in mice produces a profound effect on the response to fasting and facilitates entry into torpor (3). Polymorphisms in the corresponding gene are associated with bipolar affective disorder and major depressive disorder in women, indicating that variation in may be an important gender-specific risk factor for certain mental disorders (4,5). Alternate Names G protein-coupled receptor 50; GPR50; H9; Mel1c; Melatonin-related receptor; MGC125342; MTR1L Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 70-75 Source/Isotype: Rabbit IgG