CST, 14037S, Secretagogin (D4V1Y) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying Secretagogin. Validated for WB,IP,IHC,IF. Available in 2 sizes. Highly specific and rigorously validated in-house, Secretagogin (D4V1Y) Rabbit Monoclonal Antibody (CST #14037) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Immunohistochemistry (Paraffin): 1:3200 Immunofluorescence (Frozen): 1:800 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunohistochemistry (Paraffin), Immunofluorescence (Frozen) Specificity / Sensitivity Secretagogin (D4V1Y) Rabbit Monoclonal Antibody recognizes endogenous levels of total secretagogin protein. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the carboxy terminus of human secretagogin protein. Background Calcium-binding proteins of different subfamilies regulate the second messenger calcium. Calbindin, calmodulin, S-100, parvalbumin, and troponin C are members of the low molecular weight calcium-binding protein family (1). Calbindin is expressed in discrete neuronal populations within the CNS and is thought to act as an intracellular calcium buffering protein. Most Purkinje cells express calbindin, which is expressed when neurons start to migrate and differentiate. In contrast, other calcium buffering proteins, such as parvalbumin, are expressed later during development and in parallel with increasing neuronal activity (2). Secretagogin (SCGN) is a cytosolic calcium-binding protein with homology to calbindin-D28k (CALB1) and calretinin (CALB2). The secretagogin protein was isolated from pancreatic islets of Langerhans and insulinomas, and shows abundant pancreatic expression and reduced expression in thyroid, adrenal medulla, stomach, small intestine, and colon (3). Cloning and sequence analysis of the secretagogin cDNA indicate that the corresponding protein includes six, EF-hand calcium-binding domain tandem repeats (3). Interaction between secretagogin and vesicle trafficking proteins, including SNAP25, SNAP23, ARFGAP2, and DOC2A, suggests a possible role for SCGN in neurotransmitter and hormone release (4,5). Research studies examining secretagogin expression in fetal and developing brains point to a role for this calcium-binding protein in differentiation, brain wiring, and neuronal development (6). Alternate Names calbindin-like; CALBL; DJ501N12.8; SCGN; SECRET; Secretagogin; secretagogin, EF-hand calcium binding protein; SEGN; setagin Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 28 Source/Isotype: Rabbit IgG