{"product_id":"cst-14069t","title":"CST,  14069T, ETS-1 (D8O8A) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying Ets-1. Validated for WB,IP,IF,ChIP,ChIP,C\u0026amp;R,C\u0026amp;T. Available in 2 sizes. Highly specific and rigorously validated in-house, ETS-1 (D8O8A) Rabbit Monoclonal Antibody (CST #14069) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nFor optimal ChIP and ChIP-seq results, use 10 μl of antibody and 10 μg of chromatin (approximately 4 x 10 6 cells) per IP. This antibody has been validated using SimpleChIP ® Enzymatic Chromatin IP Kits. The CUT\u0026amp;RUN dilution was determined using the CUT\u0026amp;RUN Assay Kit # 86652 . The CUT\u0026amp;Tag dilution was determined using CUT\u0026amp;Tag Assay Kit # 77552 .\nWestern Blotting: 1:2000\nImmunoprecipitation: 1:50\nImmunofluorescence (Immunocytochemistry): 1:3200 - 1:6400\nChromatin IP: 1:50\nChromatin IP-seq: 1:50\nCUT\u0026amp;RUN: 1:50\nCUT\u0026amp;Tag: 1:50\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 98536 .\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Immunoprecipitation, Immunofluorescence (Immunocytochemistry), Chromatin IP, Chromatin IP-seq, CUT\u0026amp;RUN, CUT\u0026amp;Tag\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nETS-1 (D8O8A) Rabbit Monoclonal Antibody recognizes endogenous levels of total ETS-1 protein.\nSpecies Reactivity: Human, Mouse, Rat\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser179 of human ETS-1 protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nETS-1 is a proto-oncoprotein that belongs to the E26 Transformation-specific Sequence (ETS) family of transcription factors that share a unique and highly conserved DNA binding domain (1). ETS-1 plays important roles in vascular development and angiogenesis (2), and vascular inflammation and remodeling (3). The target genes of ETS-1 include receptor tyrosine kinases, MMPs, and cell adhesion molecules (4-6). In addition, ETS-1 is involved in regulation of energy metabolism in cancer cells (7). ETS-1 activity is regulated by two different types of phosphorylation sites. While phosphorylation at a cluster of serine residues in the exon VII domain by CaMKII inhibits ETS-1 DNA binding activity (8), phosphorylation at its Thr38 site by Ras activates ETS-1 (9).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nAvian erythroblastosis virus E26 (v-ets) oncogene homolog-1; c-ets-1; ets protein; ETS proto-oncogene 1, transcription factor; ETS-1; ETS1; EWSR2; FLJ10768; p54; Protein C-ets-1; v-ets avian erythroblastosis virus E2 oncogene homolog 1; v-ets avian erythroblastosis virus E26 oncogene homolog 1; v-ets erythroblastosis virus E26 oncogene homolog 1 (avian)\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H M R\nSENSITIVITY: Endogenous\nMW (kDa): 52\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46797604192425,"sku":"14069T","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-14069t","provider":"Iright","version":"1.0","type":"link"}