CST, 14085S, PHB2 (E1Z5A) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying PHB2. Validated for Western Blotting,Immunoprecipitation,Immunofluorescence (Immunocytochemistry). Available in 2 sizes. Highly specific and rigorously validated in-house, PHB2 (E1Z5A) Rabbit Monoclonal Antibody (CST #14085) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Immunofluorescence (Immunocytochemistry): 1:200 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunofluorescence (Immunocytochemistry) Specificity / Sensitivity PHB2 (E1Z5A) Rabbit Monoclonal Antibody recognizes endogenous levels of total PHB2 protein. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human PHB2 protein. Background The prohibitins PHB1 and PHB2 are highly conserved, multifunctional proteins present in eukaryotic nuclear and mitochondrial compartments (1). Prohibitin-2 (PHB2, REA) was originally identified as an estrogen receptor-specific coregulator. PHB2 directly interacts with hormone-bound estrogen receptor and represses its transcriptional activity through competitive inhibition of Src-1 coactivation of the estrogen receptor (2,3). Together with COUP transcription factors, PHB2 interacts with histone deacetylases HDAC1 and HDAC5 to mediate transcriptional regulation by the estrogen receptor through coupling the deacetylase to the transcription activation complex (4). Prohibitin PHB1/PHB2 heterodimers form large ring complexes on the mitochondrial membrane (5) and act as chaperones to stabilize mitochondrial proteins, such as OPA1 and Hax1, to support mitochondrial morphogenesis and protect against apoptosis (6-8). Alternate Names B-cell associated protein; B-cell receptor-associated protein BAP37; BAP; Bap37; BCAP37; D-prohibitin; hBAP; MGC117268; p22; PHB2; PNAS-141; prohibitin 2; Prohibitin-2; REA; Repressor of estrogen receptor activity Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 33 Source/Isotype: Rabbit IgG