{"product_id":"cst-14090s","title":"CST,  14090S, APC11 (D1E7Q) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying APC11. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, APC11 (D1E7Q) Rabbit Monoclonal Antibody (CST #14090) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\nImmunoprecipitation: 1:100\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Immunoprecipitation\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nAPC11 (D1E7Q) Rabbit Monoclonal Antibody recognizes endogenous levels of total APC11 protein. This antibody does not cross-react with either RBX1 or RBX2.\nSpecies Reactivity: Human, Mouse, Rat, Monkey\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a full-length human recombinant APC11 protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nEukaryotic cell proliferation depends strictly upon the E3 ubiquitin ligase activity of the anaphase promoting complex\/cyclosome (APC\/C), whose main function is to trigger the transition of the cell cycle from metaphase to anaphase. The APC\/C complex promotes the assembly of polyubiquitin chains on substrate proteins in order to target these proteins for degradation by the 26S proteasome (1,2). The vertebrate APC\/C complex consists of as many as 15 subunits, including multiple scaffold proteins, two catalytic subunits (APC2, APC11), and a number of proteins responsible for substrate recognition (3). All E3 enzymes, including APC\/C, utilize ubiquitin residues activated by E1 enzymes and transferred to E2 enzymes. Research studies indicate that APC\/C interacts with the E2 enzymes UBE2S and UBE2C via the RING-finger domain-containing subunit APC11 (4-6). APC\/C function relies on multiple cofactors, including an APC\/C coactivator formed by the cell division control protein 20 homolog (CDC20) and Cdh1\/FZR1. The CDC20\/Cdh1 coactivator is responsible for recognition of APC\/C substrates through interaction with specific D-box and KEN-box recognition elements within these substrates (7-9). Anaphase-promoting complex subunit 11 (APC11) harbors a RING-H2 motif, which is characterized by a series of non-tandem His and Cys residues responsible for the coordination of zinc cations. At the primary amino acid level, APC11 displays sequence similarity to RING-box proteins RBX1 and RBX2, which are the RING-H2 motif-containing subunits of SCF ubiquitin ligase complexes (10). A heterodimer complex containing APC11 and the cullin-like subunit, APC2, forms the catalytic core of the APC\/C and is critical for the APC\/C to catalyze ubiquitin chain elongation (4,11).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nANAPC11; anaphase promoting complex subunit 11; anaphase promoting complex subunit 11 (yeast APC11 homolog); Anaphase-promoting complex subunit 11; APC11; APC11 anaphase promoting complex subunit 11 homolog; Apc11p; Cyclosome subunit 11; Hepatocellular carcinoma-associated RING finger protein; HSPC214; MGC882\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H M R Mk\nSENSITIVITY: Endogenous\nMW (kDa): 10\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46797604389033,"sku":"14090S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-14090s","provider":"Iright","version":"1.0","type":"link"}