CST, 14108S, GIMAP5 Antibody

Polyclonal Antibody for studying GIMAP5. Validated for Western Blotting,Immunoprecipitation. Highly specific and rigorously validated in-house, GIMAP5 Antibody (CST #14108) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity GIMAP5 Antibody recognizes endogenous levels of total GIMAP5 protein. This antibody also recognizes proteins of unknown origin at 12 kDa and 50 kDa. Species Reactivity: Human Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human GIMAP5 protein. Antibodies are purified by protein A and peptide affinity chromatography. Background GTPase immune-associated proteins (GIMAP), also known as immune-associated nucleotide-binding (IAN) proteins, are evolutionarily conserved GTP-binding proteins involved in lymphocyte development, inflammation, and autoimmune diseases (reviewed in 1,2). Human GTPase IMAP family member 5 (GIMAP5, hIan5) is the homolog of the rat Ian4 protein that is mutated in severe cases of T-cell lymphopenia and insulin-dependent diabetes in Biobreeding diabetes-prone (BB-DP) rats (3,4). GIMAP5 protein is preferentially expressed in CD4- and CD8-positive T-cells as well as B-cell lymphomas (4). Research studies using GIMAP5-deficient mice show that GIMAP5 protein is critical for survival of peripheral T-cells, hematopoietic stem cells, and progenitor cells (5-7). Additional studies indicate that GIMAP5 deficiency leads to a loss of immunological tolerance (8). Polymorphisms in the human gene are associated with systemic lupus erythematosus and type I diabetes (9-11). Potential mechanisms for GIMAP5 control of cell survival include regulation of Bcl-2 family proteins, mitochondrial integrity, lysosomal function, and calcium regulation (5,7, 11-14). Alternate Names FLJ11296; GIMA5; GIMAP5; GTPase IMAP family member 5; GTPase, IMAP family member 5; hIAN5; HIMAP3; IAN-5; IAN4; IAN4L1; IAN5; IMAP3; immune associated nucleotide 4 like 1; Immune-associated nucleotide-binding protein 5; immunity associated protein 3; Immunity-associated nucleotide 4-like 1 protein; Immunity-associated nucleotide 5 protein; Immunity-associated protein 3; inhibitor of radiation- and OA-induced apoptosis, Irod/Ian5; IROD Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 30,32 SOURCE: Rabbit