CST, 14138S, Twinfilin-1 (D7C6J) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying A6. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, Twinfilin-1 (D7C6J) Rabbit Monoclonal Antibody (CST #14138) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity Twinfilin-1 (D7C6J) Rabbit Monoclonal Antibody recognizes endogenous levels of total twinfilin protein. In some cell lines, this antibody also recognizes a 48 kDa band of unknown origin. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu222 of human twinfilin-1 protein. Background Twinfilin is an actin monomer-binding protein found in all eukaryotes (1). Mammals have three isoforms. Twinfilin-1 and twinfilin-2a are expressed in most non-muscle cell types, whereas twinfilin-2b is the main isoform in adult heart and skeletal muscle (2). Twinfilins are composed of two ADF-homology domains connected by a 30 kDa linker region. All twinfilins have been shown to form a 1:1 complex with G-actin, but not F-actin (reviewed in 3). Twinfilin-1 was originally known as A6 protein tyrosine kinase and thought to be part of a novel class of protein kinases. However, the protein was renamed after further studies showed no evidence of tyrosine kinase activity (4). Twinfilin-1 helps to prevent the actin filament assembly by forming a complex with actin monomers and, in mammals, has been shown to cap the filament barbed ends. It has been suggested that this regulates cell motility (5). Suppression of twinfilin-1 has also been shown to slow lymphoma cell migration to lymph nodes (6). Alternate Names A6; A6 protein tyrosine kinase; MGC23788; MGC41876; Protein A6; Protein tyrosine kinase 9; PTK9; PTK9 protein tyrosine kinase 9; TWF1; twinfilin actin binding protein 1; Twinfilin-1; twinfilin, actin-binding protein, homolog 1; twinfilin, actin-binding protein, homolog 1 (Drosophila) Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 40 Source/Isotype: Rabbit IgG