CST, 14151S, Syntaxin 5 Antibody

Polyclonal Antibody for studying STX5. Validated for Western Blotting. Highly specific and rigorously validated in-house, Syntaxin 5 Antibody (CST #14151) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity Syntaxin 5 Antibody recognizes endogenous levels of total syntaxin 5 protein. This antibody also cross-reacts with an unidentified protein of 120 kDa. Species Reactivity: Human, Mouse, Rat Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly165 of human syntaxin 5 protein. Antibodies are purified by protein A and peptide affinity chromatography. Background The membrane protein syntaxin 5 (STX5) is a key component of soluble N-ethylmaleimide-sensitive factor attachment protein (SNAP) receptor (SNARE) complexes that regulate cellular protein transport, vesicle docking, and membrane fusion (1). Syntaxin 5 protein is found as a 42 kDa ("long") protein localized to the Golgi complex and endoplasmic reticulum, and a "short" 35 kDa isoform localized primarily to the Golgi (2,3). Formation of the syntaxin 5 SNARE complex, which also includes proteins Sec22B, Bet1, GOSR1, GOSR2, and Ykt6, allows for regulation of ER-to-Golgi transport, intra-Golgi transport, and endosome-to-Golgi retrograde transport (4-6). Research studies indicate that the syntaxin 5 SNARE complex also plays an essential role in autophagy following autophagosome formation. Intracellular protein transport mediated by the syntaxin 5 complex is required for transport and localized activity of lysosomal proteases. The experimental reduction or deletion of syntaxin 5 complex components results in non-functional lysosomes and accumulation of autophagosomes (7). Alternate Names SED5; STX5; STX5A; syntaxin 5; syntaxin 5A; Syntaxin-5 Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 35, 42 SOURCE: Rabbit