CST, 14154T, PABP2 (D6F3Q) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying PABP2. Validated for Western Blotting,Immunoprecipitation,eCLIP. Available in 2 sizes. Highly specific and rigorously validated in-house, PABP2 (D6F3Q) Rabbit Monoclonal Antibody (CST #14154) is ready to ship. Product Usage Information For more information about the RBP-eCLIP service please visit Eclipsebio . Western Blotting: 1:1000 Immunoprecipitation: 1:100 eCLIP: 1:200 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity PABP2 (D6F3Q) Rabbit Monoclonal Antibody recognizes endogenous levels of total PABP2 protein. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human PABP2 protein. Background Poly(A)-binding protein 2 (PABP2, PAPBN1) is a nuclear RNA-binding protein involved in the post-transcriptional processing of RNA molecules (1). The PABP2 protein enhances RNA polyadenylation by stimulating poly(A) polymerase (PAP) activity and facilitating the interaction between PAP and the cleavage and specificity factor (CPSF) to regulate poly(A) tail length (2-4). The role that PABP2 plays in regulating poly(A) tail formation and site selection may be important in influencing the length of the 3' untranslated region (UTR), which can alter transcript stability and translation by RNA binding proteins and miRNAs (1,5). Mutations in the corresponding gene can result in oculopharyngeal muscular dystrophy (OPMD), an autosomal dominant muscle disorder characterized by weakness in proximal limb muscles, ptosis, and dysphagia (1,6). Alternate Names Nuclear poly(A)-binding protein 1; OPMD; PAB2; PABII; PABP-2; PABP2; PABPN1; poly(A) binding protein 2; poly(A) binding protein II; poly(A) binding protein nuclear 1; poly(A) binding protein, nuclear 1; Poly(A)-binding protein 2; Poly(A)-binding protein II; Polyadenylate-binding nuclear protein 1; Polyadenylate-binding protein 2 Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 50 Source/Isotype: Rabbit IgG