CST, 14159S, EAF2 (E1R8C) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying EAF2. Validated for Western Blotting,Immunoprecipitation,Chromatin IP. Available in 2 sizes. Highly specific and rigorously validated in-house, EAF2 (E1R8C) Rabbit Monoclonal Antibody (CST #14159) is ready to ship. Product Usage Information For optimal ChIP results, use 5 μl of antibody and 10 μg of chromatin (approximately 4 x 10 6 cells) per IP. This antibody has been validated using SimpleChIP ® Enzymatic Chromatin IP Kits. Western Blotting: 1:1000 Immunoprecipitation: 1:100 Chromatin IP: 1:100 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation, Chromatin IP Specificity / Sensitivity EAF2 (E1R8C) Rabbit Monoclonal Antibody recognizes endogenous levels of total EAF2 protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human EAF2 protein. Background The super elongation complex (SEC) plays a critical role in regulating RNA polymerase II (RNAPII) transcription elongation (1). The SEC is composed of AFF4, AFF1/AF4, MLLT3/AF9, and MLLT1/ENL proteins. The pathogenesis of mixed lineage leukemia is often associated with translocations of the SEC subunits joined to the histone H3 Lys4 methyltransferase mixed lineage leukemia ( ) gene (1-4). The SEC has been found to contain RNAPII elongation factors eleven-nineteen lysine-rich leukemia (ELL), ELL2, and ELL3, along with the associated factors EAF1 and EAF2, which can increase the catalytic rate of RNAPII transcription , (1,2,5-7). The SEC positive transcription elongation factor b (P-TEFb) phosphorylates the carboxy-terminal domain within the largest subunit of RNAP II at Ser2 of the heptapeptide repeat. The SEC negative transcription elongation factors, DRB-induced stimulating factor (DSIF) and negative elongation factor (NELF), signal the transition from transcription initiation and pausing to productive transcription elongation (2,8-10). The chromosomal translocation of with the members of the SEC leads to SEC recruitment to MLL regulated genes, such as the highly developmentally regulated genes, implicating the misregulation and overexpression of these genes as underlying contributors to leukemogenesis (1,2,9,11). ELL associated factor 2 (EAF2) was identified as an interacting partner of eleven-nineteen lysine-rich leukemia (ELL) (12). EAF2 (U19) has also been identified as a protein that is down-regulated in prostate cancers and exhibits growth inhibitory and tumor suppressive activity (13,14). Alternate Names BM040; EAF2; ELL associated factor 2; ELL-associated factor 2; testosterone regulated apoptosis inducer and tumor suppressor; Testosterone-regulated apoptosis inducer and tumor suppressor protein; TRAITS; U19 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 42 Source/Isotype: Rabbit IgG