CST, 14168S, Coilin (D2L3J) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying coilin. Validated for Western Blotting,Immunofluorescence (Immunocytochemistry). Available in 2 sizes. Highly specific and rigorously validated in-house, Coilin (D2L3J) Rabbit Monoclonal Antibody (CST #14168) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunofluorescence (Immunocytochemistry): 1:800 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunofluorescence (Immunocytochemistry) Specificity / Sensitivity Coilin (D2L3J) Rabbit Monoclonal Antibody recognizes endogenous levels of total coilin protein. Species Reactivity: Human, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the carboxy terminus of human coilin protein. Background The nuclear protein coilin (COIL) is found in eukaryotic nucleoplasm and serves a marker for sub-organelles known as Cajal bodies (1,2). Cajal bodies (CB) are nuclear structures that are home to various RNA-processing complexes, including those responsible for pre-mRNA splicing, processing of rRNA and histone pre-mRNA, and telomere maintenance (1-3). The presence of coilin protein is essential for CB formation, and the protein plays a role in maintaining Cajal body structural integrity (4,5). Research studies indicate that coilin binds RNA, including telomerase RNA (hTR), pre-rRNA, and U2 snRNA, in addition to DNA (5). Additional research indicates that coilin protein may exhibit specific RNase activity towards hTR and U2 snRNA transcripts, and that this activity may be regulated through phosphorylation of coilin (6). Alternate Names CLN80; COIL; Coilin; coilin p80; p80; p80-coilin Specification REACTIVITY: H Mk SENSITIVITY: Endogenous MW (kDa): 80 Source/Isotype: Rabbit IgG