CST, 14189S, Flightless-I Antibody

Polyclonal Antibody for studying Flightless-1/FLII. Validated for Western Blotting,Immunoprecipitation. Highly specific and rigorously validated in-house, Flightless-I Antibody (CST #14189) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:200 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity Flightless-I Antibody recognizes endogenous levels of total Flightless-I protein. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human Flightless-I protein. Antibodies are purified by protein A and peptide affinity chromatography. Background The ( ) gene was first identified in mutant screens for genes involved in flight behavior. Homozygous mutant alleles at the locus are embryonic lethal, whereas heterozygous mutations yield a "flightless" phenotype resulting from defects in flight muscle fiber development (1). The encoded protein (flightless-I, FLII) is a highly conserved member of the gelsolin superfamily, defined by the presence of C-terminal gelsolin motifs that function as actin-binding domains (2). Genetic knock-out studies in mice and worms confirmed that Flightless-I plays a critical and highly conserved role in embryonic development, likely through its effects on actin remodeling of the cytoskeleton (3,4). Postnatally, Flightless-I is recognized to play an important role in wound repair (5). Flightless-I protein levels are increased in many wound types, and depletion of Flightless-I protein levels has been shown to accelerate wound repair by promoting fibroblast proliferation and epithelial migration (6-8). Studies in animal models suggest that Flightless-I may inhibit the wound repair process by modulating TGF-β signaling dynamics in the wound environment (9). Alternate Names FLI; Fli1; flightless I actin binding protein; flightless I homolog; flightless I homolog (Drosophila); FLII; FLII actin remodeling protein; FLIL; MGC39265; Protein flightless-1 homolog Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 145 SOURCE: Rabbit