{"product_id":"cst-14189s","title":"CST,  14189S, Flightless-I Antibody","description":"Polyclonal Antibody for studying Flightless-1\/FLII. Validated for Western Blotting,Immunoprecipitation. Highly specific and rigorously validated in-house, Flightless-I Antibody (CST #14189) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\nImmunoprecipitation: 1:200\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Immunoprecipitation\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nFlightless-I Antibody recognizes endogenous levels of total Flightless-I protein.\nSpecies Reactivity: Human, Mouse, Rat, Monkey\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nPolyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human Flightless-I protein. Antibodies are purified by protein A and peptide affinity chromatography.\n\u003cb\u003eBackground\u003c\/b\u003e\nThe ( ) gene was first identified in mutant screens for genes involved in flight behavior. Homozygous mutant alleles at the locus are embryonic lethal, whereas heterozygous mutations yield a \"flightless\" phenotype resulting from defects in flight muscle fiber development (1). The encoded protein (flightless-I, FLII) is a highly conserved member of the gelsolin superfamily, defined by the presence of C-terminal gelsolin motifs that function as actin-binding domains (2). Genetic knock-out studies in mice and worms confirmed that Flightless-I plays a critical and highly conserved role in embryonic development, likely through its effects on actin remodeling of the cytoskeleton (3,4). Postnatally, Flightless-I is recognized to play an important role in wound repair (5). Flightless-I protein levels are increased in many wound types, and depletion of Flightless-I protein levels has been shown to accelerate wound repair by promoting fibroblast proliferation and epithelial migration (6-8). Studies in animal models suggest that Flightless-I may inhibit the wound repair process by modulating TGF-β signaling dynamics in the wound environment (9).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nFLI; Fli1; flightless I actin binding protein; flightless I homolog; flightless I homolog (Drosophila); FLII; FLII actin remodeling protein; FLIL; MGC39265; Protein flightless-1 homolog\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H M R Mk\nSENSITIVITY: Endogenous\nMW (kDa): 145\nSOURCE: Rabbit","brand":"CST","offers":[{"title":"Default Title","offer_id":46797606191273,"sku":"14189S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-14189s","provider":"Iright","version":"1.0","type":"link"}