{"product_id":"cst-14227p","title":"CST,  14227P, Phospho-p44\/42 MAPK (Erk1\/2) (Thr202\/Tyr204) (197G2) Rabbit Monoclonal Antibody (Biotinylated)","description":"Monoclonal Antibody for studying ERK1 (Thr202\/Tyr204) phosphate\/ERK2 (Thr185\/Tyr187) phosphate. Validated for Western Blotting,Peptide ELISA (DELFIA). Highly specific and rigorously validated in-house, Phospho-p44\/42 MAPK (Erk1\/2) (Thr202\/Tyr204) (197G2) Rabbit Monoclonal Antibody (Biotinylated) (CST #14227) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nBiotinylated antibodies are ideal for immunoassay technologies and high-throughput ELISA platforms that require antibody pairs where both antibodies are from the same host. Platforms utilizing biotinylated antibodies include, but are not limited to, MSD, xMAP, Quanterix Simoa, AlphaLISA, AlphaScreen, HTRF, LANCE, and TR-FRET. Optimal dilutions\/working concentrations should be determined by the end user. Please contact us if you require the antibody clone biotinylated at a different concentration, a carrier-free formulation, or a more customized packaging solution.\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 140 mM NaCl, 3 mM KCl, 10 mM sodium phosphate (pH 7.4) dibasic, 2 mM potassium phosphate monobasic, 2 mg\/mL BSA, and 50% glycerol. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nPhospho-p44\/42 MAPK (Erk1\/2) (Thr202\/Tyr204) (197G2) Rabbit Monoclonal Antibody (Biotinylated) detects endogenous levels of p44 and p42 MAP Kinase protein (Erk1 and Erk2) when dually phosphorylated at Thr202 and Tyr204 of Erk1 (Thr185 and Tyr187 of Erk2), and singly phosphorylated at Tyr204. This antibody does not cross-react with the corresponding phosphorylated residues of either JNK\/SAPK or p38 MAP kinase protein.\nSpecies Reactivity: Human, Mouse, Rat, Monkey, Mink, D. melanogaster, Zebrafish, Pig\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Thr202\/Tyr204 of human p44 MAP kinase protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nMitogen-activated protein kinases (MAPKs) are a widely conserved family of serine\/threonine protein kinases involved in many cellular programs, such as cell proliferation, differentiation, motility, and death. The p44\/42 MAPK (Erk1\/2) signaling pathway can be activated in response to a diverse range of extracellular stimuli, including mitogens, growth factors, and cytokines (1-3), and research investigators consider it an important target in the diagnosis and treatment of cancer (4). Upon stimulation, a sequential three-part protein kinase cascade is initiated, consisting of a MAP kinase kinase kinase (MAPKKK or MAP3K), a MAP kinase kinase (MAPKK or MAP2K), and a MAP kinase (MAPK). Multiple p44\/42 MAP3Ks have been identified, including members of the Raf family, as well as Mos and Tpl2\/COT. MEK1 and MEK2 are the primary MAPKKs in this pathway (5,6). MEK1 and MEK2 activate p44 and p42 through phosphorylation of activation loop residues Thr202\/Tyr204 and Thr185\/Tyr187, respectively. Several downstream targets of p44\/42 have been identified, including p90RSK (7) and the transcription factor Elk-1 (8,9). p44\/42 are negatively regulated by a family of dual-specificity (Thr\/Tyr) MAPK phosphatases, known as DUSPs or MKPs (10), along with MEK inhibitors, such as U0126 and PD98059.\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nERK; ERK-1; ERK-2; ERK1; ERK2; ERT1; ERT2; Extracellular signal-regulated kinase 1; Extracellular signal-regulated kinase 2; extracellular signal-regulated kinase-2; extracellular signal-related kinase 1; HS44KDAP; HUMKER1A; Insulin-stimulated MAP2 kinase; MAP kinase 1; MAP kinase 2; MAP kinase 3; MAP kinase isoform p42; MAP kinase isoform p44; MAPK 1; MAPK 2; MAPK 3; MAPK1; MAPK2; MAPK3; MGC20180; Microtubule-associated protein 2 kinase; Mitogen-activated protein kinase 1; Mitogen-activated protein kinase 2; Mitogen-activated protein kinase 3; MK01; MK03; p38; p40; p41; p41mapk; p42-MAPK; P42MAPK; p44-ERK1; p44-MAPK; P44ERK1; P44MAPK; PRKM1; PRKM2; PRKM3; protein tyrosine kinase ERK2\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H M R Mk Mi Dm Z Pg\nSENSITIVITY: Endogenous\nMW (kDa): 42, 44\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46800342450345,"sku":"14227P","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-14227p","provider":"Iright","version":"1.0","type":"link"}