CST, 14256S, GABARAPL2 (D1W9T) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying GABARAPL2. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, GABARAPL2 (D1W9T) Rabbit Monoclonal Antibody (CST #14256) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity GABARAPL2 (D1W9T) Rabbit Monoclonal Antibody recognizes endogenous levels of total GABARAPL2 protein. Bands of unknown origin are detected at 80 and 110 kDa in some cell lines. This antibody has a preference for the Type I form of GABARAPL2. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human GABARAPL2 protein. Background GABA receptor associated protein (GABARAP) is an Atg8 family protein with a key role in autophagy, which was originally discovered as a protein associated with the GABA receptor regulating receptor trafficking to the plasma membrane (1). Proteins in this family, including microtubule-associated protein light chain 3 (LC3) and GATE-16 (GABARAPL2), become incorporated into the autophagosomal membranes following autophagic stimuli such as starvation (2). Like the other family members, GABARAP is cleaved at its carboxyl terminus, which leads to conjugation by either of the phospholipids phosphatidylethanolamine or phosphatidylserine (3,4). This processing converts GABARAP from a type I to a type II membrane bound form involved in autophagosome biogenesis. Processing of GABARAP involves cleavage by Atg4 family members (5,6) followed by conjugation by the E1 and E2 like enzymes Atg7 and Atg3 (7,8). GABARAPL1/GEC1, a protein that is highly related to GABARAP, was identified as an estrogen inducible gene, and is also associated with autophagosomes (9-11). GABARAPL2/GATE-16 was identified as a modulator of membrane transport, interacting with N-ethylmaleimide senstive factor (NSF) and the Golgi v-SNARE GOS-28 (12). In addition, GABARAPL2 interacts with OSBP-related protein 7 (ORP7), the GTPase GIMAP6, and the calcium channel TRPML3. (13-15) Alternate Names ATG8; ATG8C; FLC3A; GABA type A receptor associated protein like 2; GABA(A) receptor-associated protein-like 2; GABARAPL2; Gamma-aminobutyric acid receptor-associated protein-like 2; Ganglioside expression factor 2; GATE-16; GATE16; GBRL2; GEF-2; GEF2; General protein transport factor p16; Golgi-associated ATPase enhancer of 16 kDa; MAP1 light chain 3 related protein; MAP1 light chain 3-related protein Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 14 Source/Isotype: Rabbit IgG