CST, 14303T, PSMD11 (D1T1R) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying PSMD11. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, PSMD11 (D1T1R) Rabbit Monoclonal Antibody (CST #14303) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity PSMD11 (D1T1R) Rabbit Monoclonal Antibody recognizes endogenous levels of total PSMD11 protein. This antibody does not cross-react with COPS2 protein. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human PSMD11 protein. Background The 26S proteasome is a highly abundant proteolytic complex involved in the degradation of ubiquitinated substrate proteins. It consists largely of two sub-complexes, the 20S catalytic core particle (CP) and the 19S/PA700 regulatory particle (RP) that can cap either end of the CP. The CP consists of two stacked heteroheptameric β-rings (β ) that contain three catalytic β-subunits and are flanked on either side by two heteroheptameric α-rings (α ). The RP includes a base and a lid, each having multiple subunits. The base, in part, is composed of a heterohexameric ring of ATPase subunits belonging to the AAA (ATPases Associated with diverse cellular Activities) family. The ATPase subunits function to unfold the substrate and open the gate formed by the α-subunits, thus exposing the unfolded substrate to the catalytic β-subunits. The lid consists of ubiquitin receptors and DUBs that function in recruitment of ubiquitinated substrates and modification of ubiquitin chain topology (1,2). Other modulators of proteasome activity, such as PA28/11S REG, can also bind to the end of the 20S CP and activate it (1,2). Proteasome 26S subunit, non-ATPase, 11 (PSMD11, RPN6) is a non-ATPase subunit of the 19S/PA700 RP lid subcomplex. Research studies have shown that PSMD11 expression is regulated by FOXO4 in human embryonic stem cells (hESCs). Increased PSMD11 expression in hESCs correlates with high levels of proteasome activity, which may be required for protection from proteotoxic stress and senescence (3). Alternate Names 26S proteasome non-ATPase regulatory subunit 11; 26S proteasome regulatory subunit 9; 26S proteasome regulatory subunit p44.5; 26S proteasome regulatory subunit RPN6; 26S proteasome regulatory subunit S9; MGC3844; p44.5; proteasome (prosome, macropain) 26S subunit, non-ATPase, 11; proteasome 26S subunit, non-ATPase 11; PSD11; PSMD11; Rpn6; S9 Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 47 Source/Isotype: Rabbit IgG