{"product_id":"cst-14304s","title":"CST,  14304S, Gephyrin Antibody","description":"Polyclonal Antibody for studying gephyrin. Validated for Western Blotting. Highly specific and rigorously validated in-house, Gephyrin Antibody (CST #14304) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nGephyrin Antibody recognizes endogenous levels of total gephyrin protein.\nSpecies Reactivity: Human, Mouse, Rat\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nPolyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu517 of human gephyrin protein. Antibodies are purified by protein A and peptide affinity chromatography.\n\u003cb\u003eBackground\u003c\/b\u003e\nThe neurotransmitters GABA and glycine activate ligand-gated chloride channels and thus mediate fast synaptic inhibition. Gephyrin is a postsynaptic, scaffolding protein anchoring type A GABA and glycine receptors to the cytoskeleton. In addition to gephyrin's function of clustering synaptic neurotransmitter receptors, it plays an essential role in the biosynthesis of the molybdenum cofactor (MoCo). Molybdenum cofactor chelates and activates sulfite oxidase, an enzyme crucial for survival (1). GSK-3β and Erk1\/2 phosphorylate gephyrin at residue Ser270 and Ser268, respectively. These post-translational modifications alter the clustering of gephyrin, affecting the amplitude and frequency of GABAergic inhibitory currents (2,3). Researchers are analyzing the role of abnormal gephyrin clustering and function in major neurological, neurodevelopmental, and psychiatric disorders (1). Abnormal accumulations of gephyrin are highly correlated with the neuropathologic diagnosis of Alzheimer's disease (4).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nDomain E; Domain G; GEPH; Gephyrin; GPH; GPHN; GPHRYN; HKPX1; KIAA1385; MOCODC; Molybdopterin adenylyltransferase; Molybdopterin molybdenumtransferase; MPT adenylyltransferase; MPT Mo-transferase\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H M R\nSENSITIVITY: Endogenous\nMW (kDa): 93\nSOURCE: Rabbit","brand":"CST","offers":[{"title":"Default Title","offer_id":46797607501993,"sku":"14304S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-14304s","provider":"Iright","version":"1.0","type":"link"}