CST, 14309S, Citrate Synthase (D7V8B) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying Citrate synthase. Validated for Western Blotting,Immunofluorescence (Immunocytochemistry),Flow Cytometry (Fixed/Permeabilized). Available in 2 sizes. Highly specific and rigorously validated in-house, Citrate Synthase (D7V8B) Rabbit Monoclonal Antibody (CST #14309) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunofluorescence (Immunocytochemistry): 1:50 - 1:100 Flow Cytometry (Fixed/Permeabilized): 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunofluorescence (Immunocytochemistry), Flow Cytometry (Fixed/Permeabilized) Specificity / Sensitivity Citrate Synthase (D7V8B) Rabbit Monoclonal Antibody recognizes endogenous levels of total citrate synthase protein. Species Reactivity: Human, Mouse, Rat, Hamster, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human citrate synthase protein. Background Citrate synthase (CS) is a mitochondrial enzyme that catalyzes the first and rate-limiting reaction of the citric acid cycle (1,2). The enzyme is responsible for the conversion of oxaloacetate and acetyl-CoA to citrate and CoA (1,2). Research studies show that CS knockdown HeLa cells are more malignant than vector-transfected cells from an xenograft model, which suggests a direct link between the Warburg Effect and tumor growth (2). Intervertebral disc degeneration, a growing health problem, has been linked to changes in citrate synthase activity (3). Alternate Names CISY; Citrate (Si)-synthase; citrate synthase; Citrate synthase, mitochondrial; CS Specification REACTIVITY: H M R Hm Mk SENSITIVITY: Endogenous MW (kDa): 45 Source/Isotype: Rabbit IgG