{"product_id":"cst-14309s","title":"CST,  14309S, Citrate Synthase (D7V8B) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying Citrate synthase. Validated for Western Blotting,Immunofluorescence (Immunocytochemistry),Flow Cytometry (Fixed\/Permeabilized). Available in 2 sizes. Highly specific and rigorously validated in-house, Citrate Synthase (D7V8B) Rabbit Monoclonal Antibody (CST #14309) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\nImmunofluorescence (Immunocytochemistry): 1:50 - 1:100\nFlow Cytometry (Fixed\/Permeabilized): 1:50\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Immunofluorescence (Immunocytochemistry), Flow Cytometry (Fixed\/Permeabilized)\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nCitrate Synthase (D7V8B) Rabbit Monoclonal Antibody recognizes endogenous levels of total citrate synthase protein.\nSpecies Reactivity: Human, Mouse, Rat, Hamster, Monkey\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human citrate synthase protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nCitrate synthase (CS) is a mitochondrial enzyme that catalyzes the first and rate-limiting reaction of the citric acid cycle (1,2). The enzyme is responsible for the conversion of oxaloacetate and acetyl-CoA to citrate and CoA (1,2). Research studies show that CS knockdown HeLa cells are more malignant than vector-transfected cells from an xenograft model, which suggests a direct link between the Warburg Effect and tumor growth (2). Intervertebral disc degeneration, a growing health problem, has been linked to changes in citrate synthase activity (3).Â\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nCISY; Citrate (Si)-synthase; citrate synthase; Citrate synthase, mitochondrial; CS\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H M R Hm Mk\nSENSITIVITY: Endogenous\nMW (kDa): 45\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46797607534761,"sku":"14309S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-14309s","provider":"Iright","version":"1.0","type":"link"}