{"product_id":"cst-14310s","title":"CST,  14310S, Phospho-GSK-3 beta (Ser9) (D85E12) Rabbit Monoclonal Antibody (Pacific Blue™ Conjugate)","description":"Monoclonal Antibody for studying GSK3-beta (Ser9) phosphate. Validated for Flow Cytometry (Fixed\/Permeabilized). Highly specific and rigorously validated in-house, Phospho-GSK-3 beta (Ser9) (D85E12) Rabbit Monoclonal Antibody (Pacific Blue\u003csup\u003e™\u003c\/sup\u003e Conjugate) (CST #14310) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nFlow Cytometry (Fixed\/Permeabilized): 1:50\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in PBS (pH 7.2), less than 0.1% sodium azide and 2 mg\/ml BSA. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Flow Cytometry (Fixed\/Permeabilized)\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nPhospho-GSK-3 beta (Ser9) (D85E12) Rabbit Monoclonal Antibody (Pacific Blue™ Conjugate) detects endogenous levels of GSK-3β only when phosphorylated at Ser9. This antibody conjugate reacts with denatured components of bovine serum, including BSA.\nSpecies Reactivity: Human, Mouse, Rat, Hamster, Monkey\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser9 of human GSK-3β.\n\u003cb\u003eBackground\u003c\/b\u003e\nGlycogen synthase kinase-3 (GSK-3) was initially identified as an enzyme that regulates glycogen synthesis in response to insulin (1). GSK-3 is a ubiquitously expressed serine\/threonine protein kinase that phosphorylates and inactivates glycogen synthase. GSK-3 is a critical downstream element of the PI3K\/Akt cell survival pathway whose activity can be inhibited by Akt-mediated phosphorylation at Ser21 of GSK-3α and Ser9 of GSK-3β (2,3). GSK-3 has been implicated in the regulation of cell fate in and is a component of the Wnt signaling pathway required for and mammalian development (4). GSK-3 has been shown to regulate cyclin D1 proteolysis and subcellular localization (5).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nglycogen synthase kinase 3 beta; Glycogen synthase kinase-3 beta; GSK-3 beta; GSK3B; GSK3beta; GSK3beta isoform; Serine\/threonine-protein kinase GSK3B\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H M R Hm Mk\nSENSITIVITY: Endogenous\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46800342941865,"sku":"14310S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-14310s","provider":"Iright","version":"1.0","type":"link"}