CST, 14312T, E4BP4/NFIL3 (D5K8O) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying E4BP4. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, E4BP4/NFIL3 (D5K8O) Rabbit Monoclonal Antibody (CST #14312) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:100 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity E4BP4/NFIL3 (D5K8O) Rabbit Monoclonal Antibody recognizes endogenous levels of total E4BP4/NFIL3 protein. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro455 of human E4BP4/NFIL3 protein. Background E4BP4/NFIL3 is a basic leucine zipper transcriptional regulator that plays a variety of roles in the immune system (1). For example, E4BP4/NFIL3 is required for bone marrow-derived NK cell development (2,3). In addition, it regulates IgE class switching in B cells by controlling IL-4 dependent induction of germ-line ε (GLε) transcription (4). In macrophages, E4BP4/NFIL3 is induced following exposure to bacteria and negatively regulates transcription of IL-12 p40. E4BP4/NFIL3 also controls cytokine production by T cells. Th2 cells from E4BP4/NFIL3 mice produced elevated levels of IL-5 an IL-13, but reduced levels of IL-4 ad IL-10 (5,6). Chronically stimulated Th1 cells, regulatory T cells, and NKT cells from E4BP4/NFIL3 mice all produce reduced levels of IL-10 and IL-13 (6). Finally, E4BP4/NFIL3 is also required for the development of conventional CD8α dendritic cells, as this subset is absent from E4BP4/NFIL3 mice (7). Alternate Names adenovirus E4 promoter region binding protein; E4 promoter-binding protein 4; E4BP4; IL3BP1; Interleukin-3 promoter transcriptional activator; Interleukin-3-binding protein 1; NF-IL3A; NFIL3; NFIL3A; Nuclear factor interleukin-3-regulated protein; nuclear factor, interleukin 3 regulated; Transcriptional activator NF-IL3A Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 60 Source/Isotype: Rabbit IgG