{"product_id":"cst-14312t","title":"CST,  14312T, E4BP4\/NFIL3 (D5K8O) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying E4BP4. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, E4BP4\/NFIL3 (D5K8O) Rabbit Monoclonal Antibody (CST #14312) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\nImmunoprecipitation: 1:100\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Immunoprecipitation\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nE4BP4\/NFIL3 (D5K8O) Rabbit Monoclonal Antibody recognizes endogenous levels of total E4BP4\/NFIL3 protein.\nSpecies Reactivity: Human, Mouse, Rat\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro455 of human E4BP4\/NFIL3 protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nE4BP4\/NFIL3 is a basic leucine zipper transcriptional regulator that plays a variety of roles in the immune system (1). For example, E4BP4\/NFIL3 is required for bone marrow-derived NK cell development (2,3). In addition, it regulates IgE class switching in B cells by controlling IL-4 dependent induction of germ-line ε (GLε) transcription (4). In macrophages, E4BP4\/NFIL3 is induced following exposure to bacteria and negatively regulates transcription of IL-12 p40. E4BP4\/NFIL3 also controls cytokine production by T cells. Th2 cells from E4BP4\/NFIL3 mice produced elevated levels of IL-5 an IL-13, but reduced levels of IL-4 ad IL-10 (5,6). Chronically stimulated Th1 cells, regulatory T cells, and NKT cells from E4BP4\/NFIL3 mice all produce reduced levels of IL-10 and IL-13 (6). Finally, E4BP4\/NFIL3 is also required for the development of conventional CD8α dendritic cells, as this subset is absent from E4BP4\/NFIL3 mice (7).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nadenovirus E4 promoter region binding protein; E4 promoter-binding protein 4; E4BP4; IL3BP1; Interleukin-3 promoter transcriptional activator; Interleukin-3-binding protein 1; NF-IL3A; NFIL3; NFIL3A; Nuclear factor interleukin-3-regulated protein; nuclear factor, interleukin 3 regulated; Transcriptional activator NF-IL3A\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H M R\nSENSITIVITY: Endogenous\nMW (kDa): 60\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46797607764137,"sku":"14312T","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-14312t","provider":"Iright","version":"1.0","type":"link"}