{"product_id":"cst-14323s","title":"CST,  14323S, Spi-B (D3C5E) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying SPIB. Validated for Immunoprecipitation,Immunofluorescence (Immunocytochemistry),Flow Cytometry (Fixed\/Permeabilized). Available in 2 sizes. Highly specific and rigorously validated in-house, Spi-B (D3C5E) Rabbit Monoclonal Antibody (CST #14323) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nImmunoprecipitation: 1:100\nImmunofluorescence (Immunocytochemistry): 1:200\nFlow Cytometry (Fixed\/Permeabilized): 1:100 - 1:400\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Immunoprecipitation, Immunofluorescence (Immunocytochemistry), Flow Cytometry (Fixed\/Permeabilized)\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nSpi-B (D3C5E) Rabbit Monoclonal Antibody recognizes endogenous levels of total Spi-B protein.\nSpecies Reactivity: Human\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala86 of human Spi-B protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nSpi-B belongs to the ETS family of transcription factors and activates transcription through direct binding to the PU box (1). Spi-B was identified based on its homology with PU.1\/Spi-I (1). The function of Spi-B is most well studied in B cells and plasmacytoid dendritic cells (pDCs). B cells from mice deficient in Spi-B proliferate poorly and die in response to signaling through the B cell receptor (2). In addition, Spi-B mice have a defect in germinal center formation (2). Spi-B plays a role in both the development and function of pDCs. It is highly expressed by pDCs and not by monocyte-derived DCs, and overexpression of Spi-B in hematopoietic progenitor cells inhibits development of other lymphoid lineages (3). pDCs from Spi-B mice produce less interferon in response to TLR7 and TLR9 stimulation and have lower expression of pDC-specific surface markers (4). Finally, Spi-B is required for differentiation of intestinal microfold cells (5,6).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nSPI-B; Spi-B transcription factor; Spi-B transcription factor (Spi-1\/PU.1 related); SPIB; Transcription factor Spi-B\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H\nSENSITIVITY: Endogenous\nMW (kDa): 44\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46800246997161,"sku":"14323S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-14323s","provider":"Iright","version":"1.0","type":"link"}