CST, 14326S, TRIM5-alpha (D6Z8L) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying TRIM5. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, TRIM5-alpha (D6Z8L) Rabbit Monoclonal Antibody (CST #14326) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity TRIM5-alpha (D6Z8L) Rabbit Monoclonal Antibody recognizes endogenous levels of total TRIM5α protein. This antibody does not react with human TRIM5β and is not predicted to react with other human TRIM5 isoforms based on the location of the antigen. Species Reactivity: Human, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro395 of human TRIM5α protein. Background TRIM5α is a retroviral restriction factor that was originally identified as an HIV restriction factor in Old World monkeys (1). The restriction specificity of TRIM5α varies between species (2). Human TRIM5α only weakly restricts HIV, but efficiently restricts N-tropic murine leukemia virus (N-MLV) (1-3). TRIM5α is composed of a tripartite motif containing RING, B-box 2, and coiled-coil domains, and a B30.2/SPRY domain (4). A 13 amino acid stretch of the B30.2/SPRY domain containing multiple positively charged residues was found to be essential for viral restriction and responsible for variations across species in restriction specificity (4,5). TRIM5α blocks viral infection by interacting with the incoming viral capsid and promoting its premature disassembly (1,6,7). In addition, TRIM5α, together with UBC13-UEV1A, promotes innate immune signaling by catalyzing the synthesis of K63-linked ubiquitin chains that activate TAK1, AP-1, and NF-κB (8). Alternate Names RING finger protein 88; RING-type E3 ubiquitin transferase TRIM5; RNF88; TRIM5; TRIM5alpha; tripartite motif containing 5; tripartite motif containing 5 transcript variant iota; tripartite motif containing 5 transcript variant kappa; tripartite motif protein TRIM; tripartite motif protein TRIM5; tripartite motif-containing 5; Tripartite motif-containing protein 5 Specification REACTIVITY: H Mk SENSITIVITY: Endogenous MW (kDa): 56 Source/Isotype: Rabbit IgG