CST, 14328S, GCKR (D1W9P) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying GCKR. Validated for Western Blotting,Immunoprecipitation,Immunofluorescence (Frozen). Available in 2 sizes. Highly specific and rigorously validated in-house, GCKR (D1W9P) Rabbit Monoclonal Antibody (CST #14328) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:100 Immunofluorescence (Frozen): 1:100 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunofluorescence (Frozen) Specificity / Sensitivity GCKR (D1W9P) Rabbit Monoclonal Antibody recognizes endogenous levels of total GCKR protein. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human GCKR protein. Background Glucokinase regulatory protein (GCKR, GKRP) regulates the activity and localization of glucokinase, an important metabolic regulator of glucose uptake, glycogen synthesis, and glucose production in hepatocytes (1). GKRP plays a key role in glucose homeostasis as it inhibits glucokinase activity and sequesters this metabolic enzyme in hepatocyte nuclei; in response to changes in glucose concentration, GCKR promotes the release of glucokinase into the cytoplasm (2). During conditions of low blood glucose levels, GCKR binds to fructose-6-phosphate, which leads to GCKR binding to glucokinase and the subsequent nuclear localization and inactivation of glucokinase. After feeding, GCKR binds fructose-1-phosphate, which disrupts the interaction between GCKR and glucokinase and releases active glucokinase to cytoplasm (1-3). Polymorphisms in the corresponding gene are associated with atypical plasma triglyceride levels, fasting glucose and insulin levels, and glucokinase activity (4,5). Alternate Names FGQTL5; GCKR; GKRP; glucokinase (hexokinase 4) regulator; glucokinase (hexokinase 4) regulatory protein; Glucokinase regulator; Glucokinase regulatory protein; hexokinase 4; hexokinase 4 regulator Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 66 Source/Isotype: Rabbit IgG