{"product_id":"cst-14328s","title":"CST,  14328S, GCKR (D1W9P) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying GCKR. Validated for Western Blotting,Immunoprecipitation,Immunofluorescence (Frozen). Available in 2 sizes. Highly specific and rigorously validated in-house, GCKR (D1W9P) Rabbit Monoclonal Antibody (CST #14328) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\nImmunoprecipitation: 1:100\nImmunofluorescence (Frozen): 1:100\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Immunoprecipitation, Immunofluorescence (Frozen)\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nGCKR (D1W9P) Rabbit Monoclonal Antibody recognizes endogenous levels of total GCKR protein.\nSpecies Reactivity: Human, Mouse, Rat\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human GCKR protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nGlucokinase regulatory protein (GCKR, GKRP) regulates the activity and localization of glucokinase, an important metabolic regulator of glucose uptake, glycogen synthesis, and glucose production in hepatocytes (1). GKRP plays a key role in glucose homeostasis as it inhibits glucokinase activity and sequesters this metabolic enzyme in hepatocyte nuclei; in response to changes in glucose concentration, GCKR promotes the release of glucokinase into the cytoplasm (2). During conditions of low blood glucose levels, GCKR binds to fructose-6-phosphate, which leads to GCKR binding to glucokinase and the subsequent nuclear localization and inactivation of glucokinase. After feeding, GCKR binds fructose-1-phosphate, which disrupts the interaction between GCKR and glucokinase and releases active glucokinase to cytoplasm (1-3). Polymorphisms in the corresponding gene are associated with atypical plasma triglyceride levels, fasting glucose and insulin levels, and glucokinase activity (4,5).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nFGQTL5; GCKR; GKRP; glucokinase (hexokinase 4) regulator; glucokinase (hexokinase 4) regulatory protein; Glucokinase regulator; Glucokinase regulatory protein; hexokinase 4; hexokinase 4 regulator\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H M R\nSENSITIVITY: Endogenous\nMW (kDa): 66\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46797607862441,"sku":"14328S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-14328s","provider":"Iright","version":"1.0","type":"link"}