{"product_id":"cst-14340s","title":"CST,  14340S, Caspase-11 (17D9) Rat Monoclonal Antibody","description":"Monoclonal Antibody for studying CASP4 mouse. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, Caspase-11 (17D9) Rat Monoclonal Antibody (CST #14340) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\nImmunoprecipitation: 1:100\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Immunoprecipitation\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nCaspase-11 (17D9) Rat Monoclonal Antibody recognizes endogenous levels of total caspase-11 protein.\nSpecies Reactivity: Mouse\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with recombinant protein specific to the p30 subunit terminus of mouse caspase-11 protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nCaspases are a family of aspartate-specific cysteine-dependent proteases that play a critical role in apoptosis as well as inflammatory responses. Pro-inflammatory caspases include caspase-1 and mouse caspase-11 (1). Caspase-11 has about 60% and 55% identity to human caspases-4 and -5, respectively. Caspase-1 cleaves inflammatory cytokines such as pro-IL-1β and IL-18 into their mature forms (2). It is activated by proteolytic cleavage producing a tetramer of its two active subunits, p20 and p10. Canonical activation of caspase-1 occurs through several complex molecular platforms designated \"inflammasomes\" that include Pycard\/Asc, nucleotide-binding oligomerization receptors (NLRs), and AIM2 (3, 4). Non-canonical activation of caspase-1 is triggered by caspase-11, which is transcriptionally induced by toll-like receptor ligands including LPS. Activation of this pathway induces inflammatory cytokines as well as pyroptosis, a form of programmed cell death (5-9).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nCas; CASP-11; CASP-4; Casp11; Casp4; Caspa; caspase 11, apoptosis-related cysteine peptidase; caspase 11, apoptosis-related cysteine protease; caspase 4, apoptosis-related cysteine peptidase; caspase 4, apoptosis-related cysteine protease; Caspase-11; caspase-11 short form splicing; Caspase-4; Caspase-4 subunit p10; Caspase-4 subunit p20; Caspase11; Caspase4; Caspl; ich-; ich-3; Ich3; Protease ICH-3\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: M\nSENSITIVITY: Endogenous\nMW (kDa): 38, 43\nSource\/Isotype: Rat IgG2a","brand":"CST","offers":[{"title":"Default Title","offer_id":46801047552169,"sku":"14340S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-14340s","provider":"Iright","version":"1.0","type":"link"}