CST, 14351T, MMP-3 (D7F5B) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying MMP-3. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, MMP-3 (D7F5B) Rabbit Monoclonal Antibody (CST #14351) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity MMP-3 (D7F5B) Rabbit Monoclonal Antibody recognizes endogenous levels of total MMP-3 protein. Species Reactivity: Human, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser417 of human MMP-3 protein. Background The matrix metalloproteinases (MMPs) are a family of proteases that target many extracellular proteins including other proteases, growth factors, cell surface receptors, and adhesion molecules (1). Among the family members, MMP-2, MMP-3, MMP-7, and MMP-9 have been characterized as important factors for normal tissue remodeling during embryonic development, wound healing, tumor invasion, angiogenesis, carcinogenesis, and apoptosis (2-4). Research studies have shown that MMP activity correlates with cancer development (2). One mechanism of MMP regulation is transcriptional (5). Once synthesized, MMP exists as a latent proenzyme. Maximum MMP activity requires proteolytic cleavage to generate active MMPs by releasing the inhibitory propeptide domain from the full-length protein (5). Alternate Names CHDS6; matrix metallopeptidase 3; matrix metallopeptidase 3 (stromelysin 1, progelatinase); matrix metalloproteinase 3 (stromelysin 1, progelatinase); Matrix metalloproteinase-3; MGC126102; MGC126103; MGC126104; MMP-3; MMP3; proteoglycanase; SL-1; STMY; STMY1; STR1; Stromelysin-1; Transin-1 Specification REACTIVITY: H R SENSITIVITY: Endogenous MW (kDa): 60 Source/Isotype: Rabbit IgG