{"product_id":"cst-14358t","title":"CST,  14358T, Toll-like Receptor 4 (D8L5W) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying TLR4 mouse. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, Toll-like Receptor 4 (D8L5W) Rabbit Monoclonal Antibody (CST #14358) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 μg\/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20ºC. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nToll-like Receptor 4 (D8L5W) Rabbit Monoclonal Antibody recognizes endogenous levels of total Toll-like receptor 4 protein.\nSpecies Reactivity: Mouse\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with recombinant mouse Toll-like receptor 4 protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nMembers of the Toll-like receptor (TLR) family, named for the closely related Toll receptor in , play a pivotal role in innate immune responses (1-4). TLRs recognize conserved motifs found in various pathogens and mediate defense responses (5-7). Triggering of the TLR pathway leads to the activation of NF-κB and subsequent regulation of immune and inflammatory genes (4). The TLRs and members of the IL-1 receptor family share a conserved stretch of approximately 200 amino acids known as the Toll\/Interleukin-1 receptor (TIR) domain (1). Upon activation, TLRs associate with a number of cytoplasmic adapter proteins containing TIR domains, including myeloid differentiation factor 88 (MyD88), MyD88-adapter-like\/TIR-associated protein (MAL\/TIRAP), TIR domain-containing adapter-inducing IFN-β (TRIF), and Toll-receptor-associated molecule (TRAM) (8-10). This association leads to the recruitment and activation of IRAK1 and IRAK4, which form a complex with TRAF6 to activate TAK1 and IKK (8,11-14). Activation of IKK leads to the degradation of IκB, which normally maintains NF-κB in an inactive state by sequestering it in the cytoplasm. TLR4 functions in association with MD-2 in the recognition and initiation of immune responses elicited by lipopolysaccharides (LPS) of Gram-negative bacteria (4-8). TLR4 triggers the activation of NF-κB, IRF-3, and MAPK pathways, leading to the production of inflammatory cytokines (9).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nCD284; lipopolysaccharide response; Lps; Ly87; OTTMUSP00000000296; Ran\/M1; Ras; Rasl2-8; Tlr4; toll like receptor 4; Toll-like receptor 4\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: M\nSENSITIVITY: Endogenous\nMW (kDa): 100-135\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46801047519401,"sku":"14358T","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-14358t","provider":"Iright","version":"1.0","type":"link"}