CST, 14372S, KPNA2 Antibody

Polyclonal Antibody for studying karyopherin alpha2. Validated for Western Blotting. Highly specific and rigorously validated in-house, KPNA2 Antibody (CST #14372) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity KPNA2 Antibody recognizes endogenous levels of total KPNA2 protein. Species Reactivity: Human, Mouse, Rat Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Glu66 of human KPNA2 protein. Antibodies are purified by protein A and peptide affinity chromatography. Background Importins belong to the karyopherin family of nuclear transport proteins and are divided into two subgroups: importin alpha and importin beta. Importins function mainly in the import and export of nuclear proteins (1,2). KPNA2 (karyopherin alpha 2), a member of the importin alpha family, contains an N-terminal importin beta binding (IBB) motif followed by a hydrophobic region consisting of 10 armadillo repeats that function in binding to the nuclear localization signal (NLS) sites of cargo proteins (3-5). A trimeric complex (importin beta/KPNA2/cargo protein) forms, translocates into the nucleus, and then dissociates upon binding of RanGTP to importin beta. The dissociated importin alpha is recycled back to the cytoplasm with the help of export factor CAS (6,7). KPNA2 can differentially regulate target localization by binding to different cargo proteins, either actively transporting them to the nucleus (such as oct3/4) or retaining them in the cytoplasm by formation of incompetent complexes (such as oct6/brn2) (8). Research studies indicate that KPNA2 promotes cell proliferation and tumorigenesis. Research studies have also shown that up-regulation of KPNA2 is associated with cancer progression. Therefore, it has become a focus of biomarker research (9-13). Alternate Names IMA1; IMA2; importin alpha 1; importin alpha 2; Importin subunit alpha-1; Importin subunit alpha-2; importin-alpha-P1; IPOA1; karyopherin alpha 2; karyopherin alpha 2 (RAG cohort 1, importin alpha 1); karyopherin subunit alpha 2; Karyopherin subunit alpha-2; KPNA2; pendulin; QIP2; RAG cohort 1; RAG cohort protein 1; RCH1; SRP1; SRP1-alpha; SRP1alpha Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 58 SOURCE: Rabbit